Biology subjects

Domingo, M.

Publications and source records attributed to Domingo, M..

5 recordsLinked to original sources

bioRxiv2026

First outbreak of Lumpy Skin disease in Catalonia, Spain, 2025-2026

Lumpy skin disease (LSD) is an emerging cattle disease caused by lumpy skin disease virus (LSDV), with major impacts on the industry, being classified as a Category A disease. Although it was historically confined to Africa, LSD has expanded into the Middle East, Asia and Europe. Here, we report two LSDV genomes from the first outbreak detected in Catalonia, Spain, in October 2025. The genomes were assembled from high-throughput sequencing data generated from two homogenized skin nodules. Comparative phylogenetic analyses were performed using all available complete LSDV genomes and rpo30 gene sequences. These analyses placed the LSDV isolates detected in Catalonia within clade 1.2, closely related to the isolates recently reported in Sardinia, Italy. Our findings also support a connection between recent south-western Europe and central African strains, possibly through northern Africa, and highlight the need for more complete genomes to clarify the origin and connections among recent LSDV outbreaks.

genomics↗

bioRxiv2024

Collagen organization and structure in FLBN5-/- mice using label-free microscopy: implications for pelvic organ prolapse

Pelvic organ prolapse (POP) is a gynecological disorder described by the descent of superior pelvic organs into or out of the vagina as a consequence of disrupted muscles and tissue. A thorough understanding of the etiology of POP is limited by the availability of clinically relevant samples, restricting longitudinal POP studies on soft-tissue biomechanics and structure to POP-induced models such as fibulin-5 knockout (FBLN5-/-) mice. Despite being a principal constituent in the extracellular matrix, little is known about structural perturbations to collagen networks in the FBLN5-/- mouse cervix. We identify significantly different collagen network populations in normal and prolapsed cervical cross-sections using two label-free, nonlinear microscopy techniques. Collagen in the prolapsed mouse cervix tends to be more isotropic, and displays reduced alignment persistence via 2-D Fourier Transform analysis of images acquired using second harmonic generation microscopy. Furthermore, coherent Raman hyperspectral imaging revealed elevated disorder in the secondary structure of collagen in prolapsed tissues. Our results underscore the need for in situ multimodal monitoring of collagen organization to improve POP predictive capabilities.

bioengineering↗

bioRxiv2022

Effect of surfactants on SARS-CoV-2: Molecular Dynamics Simulations

Surfactants are commonly used as disinfection agents in personal care products against bacteria and viruses, including SARS-CoV-2. However, there is a lack of understanding of the molecular mechanisms of the inactivation of viruses by surfactants. Here, we employ coarse grain (CG) and all-atom (AA) molecular dynamics simulations to investigate the interaction between general families of surfactants and the SARS-CoV-2 virus. To this end, we considered a CG model of a full virion. Overall, we found that surfactants have only a small impact over the virus envelope, being inserted into the envelope without dissolving it or generating pores, at the conditions considered here. However, we found that surfactants may induce a deep impact on the spike protein of the virus (responsible for its infectivity), easily covering it and inducing its collapse over the envelope surface of the virus. AA simulations confirmed that both negatively and positively charged surfactants are able to extensively adsorb over the spike protein and get inserted into the virus envelope. Our results suggest that the best strategy for the design of surfactants as virucidal agents will be to focus on those strongly interacting with the spike protein.

biophysics↗

bioRxiv2021

Interaction between SARS-CoV-2 spike glycoprotein and human skin models: a molecular dynamics study

The possibility of contamination of human skin by infectious virions plays an important role in indirect transmission of respiratory viruses but little is known about the fundamental physico-chemical aspects of the virus-skin interactions. In the case of coronaviruses, the interaction with surfaces (including the skin surface) is mediated by their large glycoprotein spikes that protrude from (and cover) the viral envelope. Here, we perform all atomic simulations between the SARS-CoV-2 spike glycoprotein and human skin models. We consider an "oily" skin covered by sebum and a "clean" skin exposing the stratum corneum. The simulations show that the spike tries to maximize the contacts with stratum corneum lipids, particularly ceramides, with substantial hydrogen bonding. In the case of "oily" skin, the spike is able to retain its structure, orientation and hydration over sebum with little interaction with sebum components. Comparison of these results with our previous simulations of the interaction of SARS-CoV-2 spike with hydrophilic and hydrophobic solid surfaces, suggests that the"soft" or "hard" nature of the surface plays an essential role in the interaction of the spike protein with materials.

biophysics↗

bioRxiv2020

Who reviews for predatory journals? A study on reviewer characteristics

BackgroundWhile the characteristics of scholars who publish in predatory journals are relatively well-understood, nothing is known about the scholars who review for these journals. We aimed to answer the following questions: Can we observe patterns of reviewer characteristics for scholars who review for predatory journals and for legitimate journals? Second, how are reviews for potentially predatory journals distributed globally? MethodsWe matched random samples of 1,000 predatory journals and 1,000 legitimate journals of the Cabells Scholarly Analytics journal lists with the Publons database of review reports, using the Jaro-Winkler string metric. For reviewers of matched reviews, we descriptively analysed meta-data on reviewing and publishing behaviour. ResultsWe matched 183,743 unique Publons reviews that were claimed by 19,598 reviewers. 6,077 reviews were conducted for 1160 unique predatory journals (3.31% of all reviews). 177,666 were claimed for 6,403 legitimate journals (96.69% of all reviews). The vast majority of scholars either never or only occasionally submitted reviews for predatory journals to Publons (89.96% and 7.55% of all reviewers, respectively). Smaller numbers of scholars claimed reviews predominantly or exclusively for predatory journals (0.26% and 0.35% of all reviewers, respectively). The two latter groups of scholars are of younger academic age and have fewer publications and fewer reviews than the first two groups of scholars.Developing regions feature larger shares of reviews for predatory reviews than developed regions. ConclusionThe characteristics of scholars who review for potentially predatory journals resemble those of authors who publish their work in these outlets. In order to combat potentially predatory journals, stakeholders will need to adopt a holistic approach that takes into account the entire research workflow.

scientific communication and education↗