Biology subjects

Doll, Y.

Publications and source records attributed to Doll, Y..

3 recordsLinked to original sources

bioRxiv2026

VLCFA-mediated inter-cell layer communication controls cellular pluripotency in Arabidopsis callus

Plants have remarkable capacity to reconstruct entire organ systems from tissue explants. In Arabidopsis two-step tissue culture system, pluripotency regulators are specifically expressed in the middle-cell layer of the stratified callus tissue. However, regulatory mechanisms underlying the radial patterning of callus remained unclear. Here, we found that very-long-chain fatty acids (VLCFAs) synthesized in the epidermis-like outermost layer are essential for pluripotency acquisition and successful shoot regeneration. Our genetic and transcriptomic analyses revealed that the regulatory roles of VLCFAs on pluripotency acquisition involve inter-cell layer signaling in callus tissue, while they are at least partly independent of ATML1/PDF2 functions and cuticular wax synthesis in the outermost layer. VLCFAs spatially restrict procambium cell identity by non-cell-autonomously suppressing cytokinin signaling, thereby allowing for establishment of the middle-cell layer. We propose that the inhibitory relationships between layer-specific regulators underlie the intricate balance of cellular fate determination in pluripotent callus.

plant biology↗

bioRxiv2026

The submergence-induced drastic morphological plasticity of root in the amphibious plant Callitriche palustris

Amphibious plants can thrive in both terrestrial and submerged environments, which are fundamentally distinct. Although morphological plasticity of leaf known as heterophylly has been well investigated, the morphological plasticity of root in amphibious plants remains poorly understood. In this study, we discovered that an amphibious plant Callitriche palustris (Plantaginaceae), which has significant heterophylly, has a remarkable morphological plasticity also in root in response to submergence. This species develops thin roots with abundant root hairs, fewer cortical and epidermal cells, and smaller aerenchyma in the terrestrial condition. On the other hand, it develops thicker roots with few root hairs, more cortical and epidermal cells, and larger aerenchyma in the submerged condition. We call this morphological plasticity of root as "heterorhizy". Phytohormone perturbation experiments revealed that abscisic acid (ABA) and gibberellin regulate root hair development and root cell division respectively. We also found the possibility that heterorhizy was acquired in the genus Callitriche. Additionally, a similar form of root hair plasticity was also observed in the phylogenetically distinct amphibious species Ludwigia arcuata (Onagraceae). Furthermore, the absence of root hair development underwater and the similar structure of aerenchyma to C. palustris were broadly seen across diverse aquatic plants. This study provides new insights into the root morphological responses to submerged environments in aquatic plants.

plant biology↗

bioRxiv2023

Experimental validation of the mechanism of stomatal development diversification

Stomata are the structures responsible for gas exchange in plants. The established framework for stomatal development is based on the model plant Arabidopsis, but diverse patterns of stomatal development have been observed in other plant lineages and species. The molecular mechanisms behind these diversified patterns are still poorly understood. We recently proposed a model for the molecular mechanisms of the diversification of stomatal development based on the genus Callitriche (Plantaginaceae), according to which a temporal shift in the expression of key stomatal transcription factors SPEECHLESS and MUTE leads to changes in the behavior of meristemoids (stomatal precursor cells). In the present study, we have tried to validate this model through genetic manipulation experiments on Arabidopsis. By altering the timing of MUTE expression, we successfully generated Arabidopsis plants with early differentiation or prolonged divisions of meristemoids, as predicted by the model. The epidermal morphology of the generated lines resembled that of species with prolonged or no meristemoid divisions. Thus, the evolutionary process was reproduced via shifting MUTE expression. We also observed unexpected phenotypes, which indicated the participation of additional factors in the evolution of the patterns observed in nature. This study provides novel experimental insights into the diversification of meristemoid behaviors. HighlightGenetic manipulation in Arabidopsis uncovered how changes in temporal gene expression patterns lead to the diversification of stomatal patterns, providing new experimental insights into the evolution of stomatal development.

plant biology↗