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Ding, E.

Publications and source records attributed to Ding, E..

2 recordsLinked to original sources

Modeling Zika Virus Spread in Colombia Using Google Search Queries and Logistic Power Models

Public health agencies generally have a small window to respond to burgeoning disease outbreaks in order to mitigate the potential impact. There has been significant interest in developing forecasting models that can predict how and where a disease will spread. However, since clinical surveillance systems typically publish data with a lag of two or more weeks, there is a need for complimentary data streams that can close this gap. We examined the usefulness of Google Trends search data for analyzing the 2016 Zika epidemic in Colombia and evaluating their ability to predict its spread. We calculated the correlation and the time delay between the reported case data and the Google Trends data using variations of the logistic growth model, and showed that the data sets were systematically offset from each other, implying a lead time in the Google Trends data. Our study showed how Internet data can potentially complement clinical surveillance data and may be used as an effective early detection tool for disease outbreaks.

epidemiology

Native and engineered clifednamide biosynthesis in multiple Streptomyces spp.

Polycyclic tetramate macrolactam (PTM) natural products are produced by actinomycetes and other bacteria. PTMs are often bioactive, and the simplicity of their biosynthetic clusters make them attractive for bioengineering. Clifednamide-type PTMs from Streptomyces sp. JV178 contain a distinctive ketone group, suggesting the existence of a novel PTM oxidizing enzyme. Here, we report the new cytochrome P450 enzyme (CftA) is required for clifednamide production. Genome mining was used to identify several new clifednamide producers, some having improved clifednamide yields. Using a parallel synthetic biology approach, CftA isozymes were used to engineer the ikarugamycin pathway of Streptomyces sp. NRRL F-2890 to yield clifednamides. Further, we observed that strong CftA expression leads to the production of a new PTM, clifednamide C. We demonstrate the utility of both genome mining and synthetic biology to rapidly increase clifednamide production and identify a PTM tailoring enzyme for rational molecule design.

synthetic biology