Dickson, B. | bioRxiv Science

bioRxiv2018

Split-QF system for fine-tuned transgene expression in Drosophila

The Q-system is a binary expression system that works well across species. Here we report the development of a split-QF system that drives strong expression, is repressible by QS and inducible by quinic acid. The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines for advanced intersectional experiments, thus greatly expanding the range of possible anatomical, physiological and behavioural assays in Drosophila.

neuroscience↗

bioRxiv2017

The VT GAL4, LexA, and split-GAL4 driver line collections for targeted expression in the Drosophila nervous system

In studying the cellular interactions within complex tissues, it is extremely valuable to be able to reproducibly and flexibly target transgene expression to restricted subsets of cells. This approach is particularly valuable in studying the nervous system, with its bewildering diversity of neuronal cell types. We report here the generation of over 18,000 driver lines (the VT collection) that exploit the GAL4, LexA, and split-GAL4 systems to express transgenes in distinct and highly specific cell types in Drosophila. We document the expression patterns of over 14,000 of these lines in the adult male brain.

neuroscience↗