Dickson, A. L. | bioRxiv Science

bioRxiv2023

Spatial Metabolomics co-registered with Multiplex Phenotyping for the Evaluation of Human Kidney Tissue

A workflow has been evaluated that utilises a single tissue section to obtain spatially co-registered, molecular, and phenotypical information suitable for AI-enabled analysis. The impact of varying DESI-MSI conditions (e.g., temperature, scan rate, acquisition time) on the detection of small molecules and on tissue quality for integration into typical clinical pathology workflows assessed in human kidney.

systems biology↗

bioRxiv2022

Identification of Unusual Oxysterols Biosynthesised in Human Pregnancy by Charge-Tagging and Liquid Chromatography - Mass Spectrometry

The aim of this study was to identify sterols, oxysterols and any down-stream metabolites in placenta, umbilical cord blood plasma, maternal plasma and amniotic fluid to enhance our knowledge of the involvement of these molecules in pregnancy. We confirm the identification of 20S- hydroxycholesterol in human placenta, previously reported in a single publication, and propose a pathway from 22R-hydroxycholesterol to a C27 bile acid of probable structure 3{beta},20R,22R- trihydroxycholest-5-en-(25R)26-oic acid. The pathway is evident not only in placenta, but pathway intermediates are also found in umbilical cord plasma, maternal plasma and amniotic fluid but not non-pregnant women.

biochemistry↗