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Diaz, T.

Publications and source records attributed to Diaz, T..

2 recordsLinked to original sources
bioRxiv2026

Integrated Analysis of HeberFERON-Driven Comparative Proteomic regulation in Glioblastoma Cells U-87MG

Glioblastoma is a very aggressive brain tumor with few therapeutics options. Type I and II Interferons (IFNs) co-formulation HeberFERON has been used in cancer treatment, with promising results in high grade brain tumors. High throughput techniques in easy-to-handle models have been important to interrogate biomolecules changes, describe mechanisms and find pharmacodynamic biomarkers. This study aims to elucidate the effect of HeberFERON over the cell proteome in comparison to its individual IFNs components. Proteomic changes with HeberFERON in the glioblastoma-derived cell line U-87MG, in comparison with individual IFN-2b and IFN-{gamma}, were studied using a nanoLC instrument EasyLC coupled to Velos Pro mass spectrometer; Maxquant and Perseus were also used. Several enrichment tools, networking analysis and canSAR for drug targets were employed. Translation, RNA processing, mitotic cell cycle, cytoskeleton and chromosome organization, apoptosis, autophagy, DNA repair are enriched to limit cellular growing together with changes in immune response components, supporting HeberFERON as a multitarget treatment. This co-formulation is distinguished at modulating RNA splicing with SMN complex, cytoskeleton organization and microtubule-based movement, nuclear envelope breakdown, DNA conformational changes, and oxidative phosphorylation, with a better drawing of effects over a variety of systems inside the tumoral cell. Together with previous microarray experiment, informative genes and proteins as pharmacodynamic biomarkers for antiproliferative effects showed up (ex. STAT1/2, CENPE, ATRIP, MAP1B, LIMA1, VCP, several ribosomal, spliceosome and proteasomal complexes proteins). This study complements transcriptomic and phosphoproteomic previous experiments in this model and underscore HeberFERON as a glioblastoma therapeutic.

cancer biology↗
bioRxiv2026

Quantitative muscle color as a proxy for structural and functional characteristics during muscle remodeling in Gryllus lineaticeps

Understanding the cellular and physiological mechanisms underlying muscle remodeling requires model systems that allow rapid, reliable, and quantitative assessment of muscle state. The cricket Gryllus lineaticeps naturally undergoes non-pathological striated muscle breakdown (histolysis), making it a promising system for studying this process. However, current assessments of muscle state are largely qualitative, subjective, and poorly standardized across experiments. Here, we developed and validated a continuous, quantitative muscle color metric to objectively capture histolysis progression and functional changes in muscle. We show that this metric robustly tracks variation in muscle color across remodeling stages, including the challenging fully transparent stage, and strongly predicts protein content, mitochondrial abundance, and iron content in a muscle- and trait-specific manner. The reproducibility of these relationships across independent datasets demonstrates the generality and robustness of this approach. By providing a rapid, objective, and biologically informative proxy of muscle state, this framework not only advances the utility of G. lineaticeps as a model for muscle remodeling but also offers a strategy for exploring the cellular dynamics underlying age-related muscle diseases and disorders, addressing an increasing public health concern in aging populations.

physiology↗