Biology subjects

Dey, T.

Publications and source records attributed to Dey, T..

3 recordsLinked to original sources

bioRxiv2020

Nutritional stress alone can control in vitro tumor formation and its invasive nature

The metastatic nature is an inherent property of the tumor. However, the effect of the environmental stress conditions on tumor microcosm in the context of metastasis needs to be analyzed. This work is proposed to analyze the tumor behavior under multiple metabolic stress conditions, such as deprivation of glucose, protein, and oxygen. The spheroid proliferation rate is observed to be influenced profoundly by the stress level where minimal stress produces compact spheroid and severe stress makes unstable aggregate like structures. It is observed that the non-invasive cancer cells cannot form spheroids under extreme stress. Stress conditions influence the mRNA levels of hypoxic, angiogenic and ECM deformation specific gene clusters. Spheroid reversal assay reveals the quiescent nature of the stressed spheroids under continuous stress conditions. However, after the rescue, the stressed spheroids were found to opt for different migration modalities. Extremely stressed non-invasive spheroids display atypical sprout-like growth within the invasion matrix and severely stressed spheroids can control the migration pattern of mesenchymal stem cells. Thus, it is concluded that multiple nutritional stress influences the spheroid formation and physiology along with the conversion of a non-invasive spheroid into quasi-invasive one.

cancer biology↗

bioRxiv2019

Reversible loss of Hippocampal Function in a Mouse Model of Demyelination/Remyelination

Demyelination of axons in the central nervous system (CNS) is a hallmark of multiple sclerosis (MS) and other demyelinating diseases. Cycles of demyelination, followed by remyelination, appear in the majority of MS patients, and are associated with the onset and quiescence of disease-related symptoms, respectively. Previous studies have shown in human patients and animal models that vast demyelination is accompanied by wide-scale changes to brain activity, but details of this process are poorly understood. We use electrophysiological recordings and nonlinear imaging of fluorescence from genetically-encoded calcium indicators to monitor the activity of hippocampal neurons during demyelination and remyelination processes over a period of 100 days. We find in vitro that synaptic transmission in CA1 neurons is diminished, and in vivo both CA1 and dentate gyrus (DG) neuronal firing rates are substantially reduced during demyelination and partially recover after a short remyelination period. This new approach allows monitoring how synaptic transmission changes, induced by cuprizone diet, are affecting neuronal activity, and can potentially be used to study the effects of therapeutic interventions in protecting the functionality of CNS neurons.

neuroscience↗

bioRxiv2019

Women in Science and Technology: An Indian scenario

The concept of treating women as equal to their male counterpart became a topic of political debate in Europe during the age of enlightenment (18th Century). The battle towards equal voting rights took approximately 100 years to win, and went until the 19th Century. It was only around 1902-1920 that women got equal voting rights in prominent Western countries followed by others. Amidst the celebration of womens vote centenary throughout the world, the issue of equal rights to education and work is still waiting for the proper attention. Historically the presence of women in educational, technological and scientific fields remains mostly marginal. In this article, the current state of under-representation of women in the science and technology community is depicted, primarily highlighting the Indian scenario. It is observed that throughout India, and amongst the relatively developed countries of the world, the presence of women in highly prestigious Institutes and Universities remains negligible even in this day and age. The probable causes behind such inequality need to be analyzed, addressed and looked upon for remedial purposes.

scientific communication and education↗