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Deka, S. S.

Publications and source records attributed to Deka, S. S..

2 recordsLinked to original sources

Effect of zinc supplementation on certain serum biochemical parameters in growing pigs

Twenty-four healthy and uniform crossbred (Hampshire x Assam Local) pigs, 4 months of age, were selected to study the effect of 500 ppm Zn supplementation on certain serum biochemical parameters for a period of four months. The animals were divided into three groups (n=8/group): group C (basal diet), group T1 (basal diet + 100 ppm Zn + calcium carbonate @ 1.5% DM) and group T2 (basal diet + 500 ppm Zn). Serum samples were collected at 15-day intervals and analysed for serum Zn, copper, total serum protein, albumin, globulin, and glucose cholesterol. The serum Zn concentration in the T1 group decreased significantly (P<0.01) from day 30 until day 120 of treatment, whereas in the T2 group, the level increased significantly (P<0.01) from day 15 onwards. The serum copper concentration did not change groups. The total protein concentration in the serum showed a significant (P<0.01) increasing trend in the T2 group from day 45 of treatment, and a significant (P<0.01) decreasing trend was observed in the T1 group compared to the C group. The serum globulin concentration decreased significantly (P< 0.05) in the T1 group compared with the C and T2 groups during the treatment period. An increasing trend in glucose and cholesterol levels was recorded at T2 during the treatment period. However, decrease, (P<0.01) in glucose and cholesterol levels was recorded in the T1 group from day 30 to day 120 of treatment. The haemoglobin concentration showed a significant (p<0.01) decreasing trend in the T1 group from day 45 onwards.

physiology↗

Optimization of conditions for conjugation of outer membrane vesicles of Salmonella Typhimurium with oral delivery systems using response surface method

BackgroundOuter membrane vesicles (OMVs) released from Salmonella Typhimurium can become the effective vaccine candidate. For efficient delivery through oral route, the OMVs are conjugated with some delivery systems. The current study was aimed at optimization of conditions required for conjugation of OMVs with nano- or microparticles for maximum entrapment of OMV in terms of protein concentration. MethodsThe OMVs of Salmonella Typhimurium were conjugated under three optimum conditions of pH, temperature and ratio (nanoparticles or microparticles: OMVs) predicted by response surface method. The efficiency of conjugation was determined by entrapment of OMVs in nano/microparticles. ResultThe pH and temperature were not influential conditions in case of conjugation of OMVs with chitosan nanoparticles (Ch-NP) and poly-lactide co-glycolide microparticles (PLG-MP) while they had influence in case of poly(anhydride) nanoparticles. In case of Ch-NP and PLG-MP, the optimum ratio for maximum entrapment was found to be 1:10 and 1:9 respectively. The optimum pH and temperature was found to be 7 and 24{degrees}C respectively for conjugation of poly(anhydride) nanoparticles. The optimized conditions did not alter the protein profile and immunogenic potential of conjugated vaccines.

biochemistry↗