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Crook, J.

Publications and source records attributed to Crook, J..

3 recordsLinked to original sources

Predictive Feature Engineering for Stress Detection using Physiological Signals, A Comparative Study

This paper presents a two-stage pipeline for implicit feature engineering in time series-based physiological stress detection using electrodermal activity (EDA) signals. In the first stage, we forecast three descriptive statistics of future EDA signals over short horizons (3, 5, and 10 seconds) based on a 60-second context window. In the second stage, a lightweight linear classifier detects stress from these predicted statistics. We evaluate three forecasting architectures spanning the domain expertise spectrum: a domain-specific bidirectional long short-term memory (BiLSTM) recurrent neural network, zero-shot and fine-tuned variants of Amazon Chronos T5 time series foundation model, and the Tabular Prior-data Fitted Network (TabPFN) applied to engineered physiological features. Experiments on the publicly available Wearable Stress and Affect Detection (WESAD) dataset, comprising chest-worn multimodal physiological signals from 15 subjects under baseline and stress conditions, use subject-independent 5-fold cross-validation and show that the domain-specific BiLSTM and TabPFN achieve comparable classification performance, with mean area under the receiver operating characteristic curve (AUC) values of 0.859-0.882 and 0.863-0.883 respectively. Both remain well ahead of the Chronos variants, which yield 0.629-0.777. Chronos models quickly reach performance saturation regardless of training depth, highlighting challenges in tokenizing continuous physiological time series. The proposed approach advances implicit feature engineering for wearable stress monitoring by leveraging forecasting as a powerful inductive bias, thereby improving robustness and providing insights into the limitations of the foundation model for physiological signals.

bioengineering↗

Antiviral activity of Tecovirimat against Mpox virus clades 1a, 1b, 2a and 2b

The recent Mpox virus (MPXV) outbreak was caused by a novel and more pathogenic clade 1b virus. We compared the antiviral efficacy of Tecovirimat in cell culture, against the two ancestral clades 1a and 2a, the clade 2b that circulated in 2022, and the recent clade 1b virus. We report that Tecovirimat similarly inhibits the replication of all four MPXV clades, at nanomolar concentrations (nM). Our results suggest that Tecovirimat remains a therapeutic option against the latest clade 1b virus.

microbiology↗

The somatically generated T cell receptor CDR3a contributes to the MHC allele specificity of the T cell receptor

Mature T cells bearing {beta} T cell receptors react with foreign antigens bound to alleles of major histocompatibility complex proteins (MHC) that they were exposed to during their development in the thymus, a phenomenon known as positive selection. The structural basis for positive selection has long been debated. Here, using mice expressing one of two different T cell receptor {beta} chains and various MHC alleles, we show that positive selection-induced MHC bias of T cell receptors is affected both by the germline encoded elements of the T cell receptor and {beta} chain and, surprisingly, dramatically affected by the non germ line encoded CDR3 of the T cell receptor chain. Thus, in addition to determining specificity for antigen, the non germline encoded elements of T cell receptors may help the proteins cope with the extremely polymorphic nature of major histocompatibility complex products within the species.

immunology↗