Coto, Z. N. | bioRxiv Science

bioRxiv2026

Evolution of neurometabolic frugality in harvester ants

Despite the importance of neurometabolic costs in brain size evolution, quantitative data on brain metabolic rates are lacking. We measured ex vivo brain metabolic rates among species of the ant genus Pogonomyrmex to differentiate the roles of sociality and body size in brain evolution in a phylogenetic context. Worker body size and colony size (a proxy for social complexity) vary significantly among Pogonomyrmex species and were positively correlated. However, sociality was not a determinant of brain energetics. Worker body size strongly affected brain metabolism: 38% of resting metabolic rate was attributable to brain metabolism in species with the smallest workers, compared to 6% in species with the largest workers. More derived species had strikingly lower mass-specific brain metabolic costs, suggesting that increases in worker body size have selected for neurometabolic frugality through reductions in brain mass-specific metabolic rate. Additionally, smaller worker body sizes may require higher brain mass-specific energetic costs to achieve comparable performance by absolutely smaller brains. Our study shows that the social brain hypothesis does not explain patterns of brain size in Pogonomyrmex, but body size and evolutionary history strongly influence brain evolution in regard to both size and metabolic cost.

zoology↗

bioRxiv2023

Behavioral and Neural Resilience to Isolation in a Eusocial Insect

Social experience early in life appears to be necessary for the development of species-typical behavior. Although isolation during critical periods of maturation has been shown to impact behavior by altering gene expression and brain development in invertebrates and vertebrates, workers of some ant species appear resilient to social deprivation and other neurobiological challenges that occur during senescence or due to loss of sensory input. It is unclear if and to what degree neuroanatomy, neurochemistry, and behavior will show deficiencies if social experience in the early adult life of worker ants is compromised. We reared newly-eclosed adult workers of Camponotus floridanus under conditions of social isolation for 2 to 53 days, quantified brain compartment volumes, recorded biogenic amine levels in individual brains, and evaluated movement and behavioral performance to compare the neuroanatomy, neurochemistry, brood-care behavior, and foraging (predatory behavior) of isolated workers with that of workers experiencing natural social contact after adult eclosion. We found that the volume of the antennal lobe, which processes olfactory inputs, was significantly reduced in workers isolated for an average of 40 days, whereas the size of the mushroom bodies, centers of higher-order sensory processing, increased after eclosion and was not significantly different from controls. Titers of the neuromodulators serotonin, dopamine, and octopamine remained stable and were not significantly different in isolation treatments and controls. Brood care, predation, and overall movement were reduced in workers lacking social contact early in life. These results suggest that the behavioral development of isolated workers of C. floridanus is specifically impacted by a reduction in the size of the antennal lobe. Task performance and locomotor ability therefore appear to be sensitive to a loss of social contact through a reduction of olfactory processing ability rather than change in the size of the mushroom bodies, which serve important functions in learning and memory, or the central complex, which controls movement. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=133 SRC="FIGDIR/small/546928v2_ufig1.gif" ALT="Figure 1"> View larger version (33K): org.highwire.dtl.DTLVardef@199228aorg.highwire.dtl.DTLVardef@aacd50org.highwire.dtl.DTLVardef@18d657corg.highwire.dtl.DTLVardef@1b9eb_HPS_FORMAT_FIGEXP M_FIG O_FLOATNOVisual Abstract.C_FLOATNO Newly eclosed (callow) Camponotus floridanus minor workers were subjected to variable periods of isolation to determine whether and how diminished social experience and isolation influence the development of the brain, including compartment volumes, biogenic amine levels, and behavioral performance. C_FIG

animal behavior and cognition↗