Biology subjects

Clauw, P.

Publications and source records attributed to Clauw, P..

4 recordsLinked to original sources

bioRxiv2025

Plant genetic and root-associated microbial diversity modulate Lactuca sativa responsiveness to a soil inoculum under phosphate deficiency

O_LIMicrobial-based approaches have been proposed as a solution to decrease the use of chemical fertilizers in agriculture. Among these, the most promising candidates are arbuscular mycorrhizal fungi (AMF), with their ability to extend the root surface and absorb phosphate, and phosphate solubilizing bacteria (PSB), but their effectiveness has been shown to depend on plant genetic diversity. C_LIO_LIWith the aim of identifying genetic markers explaining plant differential responses to soil-beneficial microbes, we monitored a panel of 128 fully sequenced varieties of Lactuca sativa under controlled P starvation conditions, treated with AMF and PSB. C_LIO_LIResults showed a strong effect of the lettuce genetic variation on the plant physiological and morphological response to the inoculum. Through genome-wide association studies, we identified specific genetic regions associated with variations in leaf phosphate and shoot biomass in response to the treatment. C_LIO_LIBeyond genetic factors, we detected changes in fungal {beta}-diversity and increases in bacterial -diversity associated with phenotypic variation, and we identified 44 ASVs linked to variation in agriculturally important traits. Among these, we experimentally validated the role of six bacterial strains through both in vitro and pot experiments, in affecting the leaf phosphate concentration and plant shoot biomass. C_LIO_LIIn conclusion, we highlighted key genetic and physiological mechanisms that could play a crucial role in enhancing microbial treatments for optimizing plant phosphate management. C_LI

plant biology↗

bioRxiv2024

Metabolic enzymes moonlight as selective autophagy receptors to protect plants against viral-induced cellular damage

AbstractRNA viruses co-opt the host endomembrane system and organelles to build replication complexes for infection. How the host responds to these membrane perturbations is poorly understood. Here, we explore the autophagic response of Arabidopsis thaliana to three viruses that hijack different cellular compartments. Autophagy is significantly induced within systemically infected tissues, its disruption rendering plants highly sensitive to infection. Contrary to being an antiviral defense mechanism as previously suggested, quantitative analyses of the viral loads established autophagy as a tolerance pathway. Further analysis of one of these viruses, the Turnip Crinkle Virus (TCV) that hijack mitochondria, showed that despite perturbing mitochondrial integrity, TCV does not trigger a typical mitophagy response. Instead, TCV and Turnip yellow mosaic virus (TYMV) infection activates a distinct selective autophagy mechanism, where oligomeric metabolic enzymes moonlight as selective autophagy receptors and degrade key executors of defense and cell death such as EDS1. Altogether, our study reveals an autophagy-regulated metabolic rheostat that gauges cellular integrity during viral infection and degrades cell death executors to avoid catastrophic amplification of immune signaling.

plant biology↗

bioRxiv2022

Locally adaptive temperature response of vegetative growth in Arabidopsis thaliana

We investigated early vegetative growth of natural Arabidopsis thaliana accessions in cold, non-freezing temperatures, similar to temperatures these plants naturally encounter in fall at northern latitudes. We found that accessions from northern latitudes produced larger seedlings than accessions from southern latitudes, partly as a result of larger seed size. However, their subsequent vegetative growth when exposed to colder temperatures was slower. The difference was too large to be explained by random population differentiation, and is thus suggestive of local adaptation, a notion that is further supported by substantial transcriptome and metabolome changes in northern accessions. We hypothesize that the reduced growth of northern accessions is an adaptive response, and a consequence of reallocating resources towards cold acclimation and winter survival.

plant biology↗

bioRxiv2020

Plasticity of the primary metabolome in 241 cold grown Arabidopsis thaliana accessions and its relation to natural habitat temperature

In the present study, 241 natural accessions of Arabidopsis thaliana were grown under two different temperature regimes, 16 {degrees}C and 6 {degrees}C, and growth parameters were recorded together with metabolite profiles to investigate the natural variation in metabolic responses and growth rates. Primary metabolism and growth rates of accessions significantly differed between accessions and both growth conditions. Relative growth rates showed high correlations to specific metabolite pools. Metabolic distances based on whole metabolite profiles were built from principal component centroids between both growth setups. Genomic prediction using ridge-regression best linear unbiased prediction (rrBLUP) revealed a significant prediction accuracy of metabolite profiles in both conditions and metabolic distances, which suggests a tight relationship between genome and primary metabolome. GWAS analysis revealed significantly associated SNPs for a number of metabolites, especially for fumarate metabolism at low temperature. A highly significant correlation was observed between metabolic distances and maximum temperature in the original growth habitat between January and March. Inverse data-driven modelling revealed that metabolic pathway regulation and metabolic reaction elasticities distinguish accessions originating from warm and cold growth habitats.

plant biology↗