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Chuang, T.-C.

Publications and source records attributed to Chuang, T.-C..

2 recordsLinked to original sources

AetherXeno: an allele-resolved receptor-occupancy atlas for regulatory pharmacogenomics

Motivation: Interpreting noncoding pharmacogenomic variants requires identifying the regulatory program that may be perturbed and the direction of the effect, information not supplied by population frequency or general regulatory annotations. Results: We developed AetherXeno, an allele-resolved atlas that scores all substitutions across 8,691 experimentally supported elements for PXR, FXR and AhR and connects 17.27 million variants to evidence matched by exact allele. Across the six paired comparisons, the gain in Spearman correlation ranged from 0.039 to 0.334. Every regional bootstrap interval excluded zero. Homology exclusion preserved these findings, while motif core and liver expression enrichment supported biological organization of the score landscape. The browser, programmatic interface and bulk files expose the same versioned records from individual variants to cohort analyses. Availability and implementation: The web resource is available at https://aetherxeno.org and the versioned data at doi:10.5281/zenodo.20753827.

bioinformatics↗

Novel Tissue Mechanics-Guided Cellular Flows Enable the Evolution of Feather Follicles

Complex tissue architecture is achieved through multiple rounds of morphological transitions. Here, we analyzed cellular flows and tissue mechanics during avian skin development. We showed how novel cellular flows initiate chemo-mechanical circuits that guide epithelial protrusion, folding, invagination, and spatial cell fate specification. In the initial feather bud formation, stiff dermal condensates protrude vertically out of the locally softened epithelial sheet. As the bud elongates, it stretches the epithelial cells at the base, which mechanically activates YAP and causes the epithelial sheet to fold downward and form a stiff cylindrical wall that invaginates into the skin. This stiff epithelial tongue is essential to the compaction and formation of the tightly packed dermal papillae. These topological transformational events are mechanically interconnected, and the completion of the previous circuit initializes the next one. On the contrary, during scale development, its rigid epithelial sheet restricts dermal cell flows, preventing other further topological transformation. We generated a topological transformation model to show how the process enables the novel evolution of feather follicles.

developmental biology↗