Cherif, H. | bioRxiv Science

bioRxiv2024

Senolytic Treatment for Low Back Pain.

Senescent cells (SnCs) accumulate due to aging and external cellular stress throughout the body. They adopt a senescence-associated secretory phenotype (SASP) and release inflammatory, and degenerative factors that actively contribute to age-related diseases such as low back pain (LBP). The senolytics, o-Vanillin and RG-7112, remove senescent human intervertebral (IVD) cells and reduce SASP release, but it is not known if they can treat LBP. sparc-/- mice, with LBP, were treated orally with o-Vanillin and RG-7112 as single or combination treatments. Treatment reduced LBP and SASP factor release and removed SnCs from the IVD and spinal cord. Treatment also lowered degeneration score in the IVDs, improved vertebral bone quality, and reduced the expression of pain markers in the spinal cord. The result indicates that RG-7112 and o-Vanillin with the combination treatment providing the strongest effect are potential disease-modifying drugs for LBP and other painful disorders where cell senescence is implicated. One Sentence Summary: Senolytics drugs can reduce back pain

cell biology↗

bioRxiv2023

Metataxonomic analysis of halophilic archaea community in two geothermal springs sources in southern Tunisian Sahara

In this study, we assess the phylogenetic diversity of the halophilic archaeal community of biotechnological interest that inhabit in geothermal water in southern Tunisia. These waters are usually used for farming purposes, particularly for irrigation of the oases and greenhouses, due to the limited availability of surface freshwater resources and the arid climate. The samples processed from two separated geothermal sources in southern Tunisia based on the Illumina Miseq sequencing approach. Three samples including water, sediment, and halite-soil crust, were collected from downstream of two geothermal springs of Ksar Ghilane (KGH) and Zaouet Al Aness (ZAN) oases. Results showed that several haloarchaea-related members were identified in geothermal springs. The average taxonomic composition showed that 20 genera out of 33 were shared between the two geothermal sources with uneven distribution, where the Halogranum genus is the most represented genus with an abundance of 18.9% and 11.58% for ZAW and KGH, respectively. Several unique site-specific genera were also observed; the case of Halonotius, Halopelagius, Natronorubrum, and Haloarcula in ZAN, and Haloprofundus, Halomarina, Halovivax, Haloplanus, Natrinema, Halobium, Natronoarchaeum, Haloterrigena detected in KGH pool. We noticed that the majority of genus members are usually found in low-salinity ecosystems. The finding of this study suggests that haloarchaea-related members could survive in downstream geothermal sources, which may have resulted from the leaching of salts and minerals through drilling waters creating chemically complex saline systems.

microbiology↗