Biology subjects

Chaumeton, C.

Publications and source records attributed to Chaumeton, C..

3 recordsLinked to original sources

bioRxiv2025

A sensorimotor instability drives a locomotor transition during fish development

Animals rely on movement to survive -- to explore their environment, find food and mates and avoid danger. During development, changes in body shape, muscle strength and physiological needs drive the continuous adjustment of locomotor patterns. How these changes are orchestrated in a flexible and adaptive manner remains unknown. We explore this question in Danionella cerebrum, a miniature freshwater fish that is emerging as an important vertebrate model in systems neuroscience. We identify a clear transition in locomotion, from continuous to burst-and-coast swimming occurring around 3 weeks of age. We demonstrate that this transition is an energy saving strategy, and that it reflects an insta-bility in the sensorimotor process governing speed regulation. Rather than a preprogrammed developmental switch, it is therefore directly tied to the animal swimming strength. We confirmed this finding by manipulating sensory feedback in order to induce a similar transition at fixed developmental stages. Together, our results illustrate a dynamic interplay between body, brain, and environment during development, offering new insights into the principles governing adaptive locomotion.

biophysics↗

bioRxiv2024

Pleiotropic function of Dlx5/6 in coordinating the development of mammalian vocal and auditory organs

Acoustic communication, essential for mammalian social interactions, involves both effector (vocal) and receptor (auditory) organs that present a wide variety of morphologies between species. The molecular mechanisms supporting the harmonious diversification of effector and receptor systems along with the evolution of species-specific acoustic communication are still poorly understood. It is conceivable that common genetic pathways determine the parallel morphogenesis of vocal and auditory systems. Here, we investigated this question by generating mutant mice with targeted invalidation of Dlx5/6 genes in the otic placode and cephalic neural crest cells, contributing to the formation of the ear and vocal tract components. We show that Dlx5/6 inactivation provokes simultaneous defects of the outer, middle and inner ear and of the jaw, pharynx and larynx musculoskeletal systems. Our findings support the notion that Dlx5/6 genes played a pleiotropic role in the co-adaptation of vocal and auditory systems in mammals.

developmental biology↗

bioRxiv2023

Identification of the Intracellular Protein Targets of a Bio-active Clickable Half-Sandwich Iridium Complex by Chemical Proteomics

Identification of intracellular targets of anticancer drug candidates provides key information on their mechanism of action. Exploiting the ability of the anticancer (C^N)-chelated half-sandwich iridium(III) complexes to covalently bind proteins, click chemistry with a bioorthogonal azido probe was used to localize a phenyloxazoline-chelated iridium complex within cells and profile its interactome at the proteome-wide scale. Proteins involved in protein folding and actin cytoskeleton regulation were identified as high affinity targets. Upon iridium complex treatment, HSP90 folding activity was inhibited in vitro and major cytoskeleton disorganization was observed. We used a wide array of imaging and biochemical methods to validate selected targets and obtain a multiscale overview of the effects of this complex on live human cells. We demonstrate that it behaves as a dual agent, inducing both electrophilic and oxidative stresses in cells that account for its cytotoxicity.

biochemistry↗