Chagas, E. H. N. | bioRxiv Science

bioRxiv2022

Occurrence Of Avian Reovirus And Picobirnavirus In Wild Birds In An Environmental Protection Area In The Amazon Biome, Para, Brazil

Wild birds have great prominence on transmission of diseases to humans, mainly due to their ease of access to human population, raising concerns about the potential impact of that proximity in context of the One Health. Studies referring to circulation of avian reovirus (ARV) and picobirnavirus (PBV) in wild birds are limited, in addition to reinforcing the development of researches that describe the prevalence, characterize the variants and evaluate the potential impact of these infections on the wild ecosystem and public health. The present study reports the occurrence of ARV and PBV in wild birds collected from an environmental protection area in the Amazon biome. RT-PCR analysis showed ARV infection prevalence in 0.6% (1/155) and PBV infection in 1.29% (2/155) on the samples. ARV strain isolated in this study demonstrated more phylogenetically related to other ARVs previously circulating in poultry in the same region. The two PBV strains obtained belong to genogroup I, and showed phylogenetically related to other PBV isolated from different animal species in different geographic regions. This study is a pioneer in the detection of ARV in wild birds in Brazil and presents a report of the first occurrence of PBV in wild birds of Guira guira specie. Additional studies in wild birds are required to increase the epidemiology, origin, evolution and emergence of new viruses that may provoke problems in the context of One Health.

microbiology↗

bioRxiv2020

DETECTION AND MOLECULAR CHARACTERIZATION OF ROTAVIRUS AND PICOBIRNAVIRUS IN WILD AVIANS FROM AMAZON FOREST

The present study reports the detection and molecular characterization of rotavirus A (RVA), rotavirus D (RVD), rotavirus F (RVF), rotavirus G (RVG) and picobirnavirus (PBV) in fecal specimens of wild and exotic birds (n = 23) from different cities of Para state, which were hospitalized at Veterinary Hospital of the Federal University of Para, Brazil, between January 2018 to June 2019. The animals exhibited different clinical signs, such as diarrhea, malnutrition, dehydration and fractures. The results showed 39.1% (9/23) of positivity for RVA by RT-qPCR. Among these, one sample (1/9) for the NSP3 gene of T2 genotype was characterized. About 88.9% (8/9) for the VP7 gene belonging to G1, equine-like G3 and G6 genotypes, and 55.5% (5/9) for the VP4 gene of P[2] genotype were obtained. In the current study, approximately 4.5% of the samples (1/23) revealed coinfection for the RVA, RVD and RVF groups. Furthermore, picobirnavirus (PBV) was detected in 1 of the 23 samples tested and was classified in the Genogroup I. The findings represent the first report of the circulation of RVA, RVD, RVF, RVG and PBV genotypes in wild birds in Brazil and suggest the possible interspecies transmission of RVs and PBVs.

molecular biology↗