Biology subjects

Ceylan, E.

Publications and source records attributed to Ceylan, E..

2 recordsLinked to original sources

bioRxiv2025

Integrated Microfluidic Platform for High-Throughput Generation of Intestinal Organoids in Hydrogel Droplets

Organoid research offers valuable insights into human biology and disease, but reproducibility and scalability remain significant challenges, particularly for epithelial organoids. Here, we present an integrated microfluidic platform that addresses these limitations by enabling high-throughput generation of uniform hydrogel microparticles embedded with intestinal stem cells. Our platform includes a cell distribution system for homogenous cell encapsulation and a microfluidic oil removal module for efficient particle transfer to aqueous media. We demonstrate the successful culture and differentiation of both healthy and tumor-derived intestinal organoids within these microparticles, achieving high homogeneity and reproducibility. This integrated microfluidic approach holds promise for scalable and standardized organoid production, with potential applications in drug screening, disease modeling, and personalized medicine.

bioengineering↗

bioRxiv2024

An implantable biohybrid nerve model towards synaptic deep brain stimulation

Restoring functional vision in blind patients lacking a healthy optic nerve requires bypassing retinal circuits, ideally, by directly stimulating the visual thalamus. However, available deep brain stimulation electrodes do not provide the resolution required for vision restoration. We developed an implantable biohybrid nerve model designed for synaptic stimulation of deep brain targets. The interface combines a stretchable stimulation array with an aligned microfluidic axon guidance system seeded with neural spheroids to facilitate the development of a 3 mm long nerve-like structure. A bioresorbable hydrogel nerve conduit was used as a bridge between the tissue and the biohybrid implant. We demonstrated stimulation of spheroids within the biohybrid structure in vitro and used high-density CMOS microelectrode arrays to show faithful activity conduction across the device. Finally, implantation of the biohybrid nerve onto the mouse cortex showed that neural spheroids grow axons in vivo and remain functionally active for more than 22 days post-implantation.

bioengineering↗