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Cervenka, J.

Publications and source records attributed to Cervenka, J..

2 recordsLinked to original sources

The Global Canopy Atlas: analysis-ready maps of 3D structure for the world's woody ecosystems

Woody canopies regulate exchanges of energy, water and carbon, and their three-dimensional (3D) structure supports much of terrestrial biodiversity. Remote sensing technologies such as airborne laser scanning (ALS) now enable the 3D mapping of entire landscapes. However, we lack the large, harmonized and geographically representative ALS collections needed to build a global picture of woody ecosystem structure. To address this challenge, we developed the Global Canopy Atlas (GCA): 3,458 ALS acquisitions transformed into standardized and analysis-ready maps of canopy height and elevation at 1 m2 resolution. The GCA covers 56,554 km2 across all major biomes. 19% of this area has been scanned multiple times, and 87% of all GCA products are openly available, covering 95% of the total area. To showcase its wide range of applications, we applied the GCA in three case studies. First, we validated three global satellite-derived canopy height maps, finding poor performance at native resolution (1-30 m, R2 < 0.38) and moderate performance at 250 m resolution (R2 < 0.65). Second, analyzing global patterns in canopy gap size frequency we discovered an unexpectedly large variation of power law exponents from branch to stand level ( = 1.52 to 2.38), pointing to a fundamental scale-dependence of forest structure. Third, we developed a framework to standardize forest turnover quantification from multi-source, multi-temporal ALS. In a temperate forest in North America it revealed that 21% of canopy gaps closed within 12 years of opening and would thus be missed by infrequent monitoring. As demonstrated by these case studies, the GCA provides a novel data source for ecologists, foresters, remote sensing scientists and the ecosystem modelling community that substantially advances our ability to understand the structure and dynamics of woody ecosystems at global scales.

ecology↗

The RNA-dependent interactions of phosphatidylinositol 4,5-bisphosphate with intrinsically disordered proteins contribute to nuclear compartmentalization.

The RNA content is crucial for the formation of nuclear compartments, such as nuclear speckles and nucleoli. Phosphatidylinositol 4,5-bisphosphate (PIP2) is found in nuclear speckles, nucleoli and nuclear lipid islets and is involved in RNA polymerase I/II transcription. Intriguingly, the nuclear localization of PIP2 was also shown to be RNA-dependent. We therefore investigated whether PIP2 and RNA cooperate in the establishment of nuclear architecture. In this study, we unveiled the RNA-dependent PIP2-associated (RDPA) nuclear proteome in human cells by mass spectrometry. We found that intrinsically disordered regions (IDRs) with polybasic PIP2-binding K/R motifs are prevalent features of RDPA proteins. Moreover, these IDRs of RDPA proteins exhibit enrichment for phosphorylation, acetylation and ubiquitination sites. Our findings reveal that RDPA protein BRD4 associates with PIP2 in an RNA-dependent manner via electrostatic interactions, and that elevated PIP2 levels increase the number of BRD4 protein nuclear foci. Thus, we propose that PIP2 spatiotemporally orchestrates nuclear processes through association with RNA and RDPA proteins and affects their ability to phase separate. This suggests pivotal role of PIP2 for the establishment of a functional nuclear architecture competent for gene expression.

cell biology↗