Biology subjects

Cano-Delgado, A. I.

Publications and source records attributed to Cano-Delgado, A. I..

5 recordsLinked to original sources

bioRxiv2025

The Arabidopsis dehydrin ERD14 chaperones the BRL3 receptor to confer drought resistance

Brassinosteroid plant hormones are involved in growth, biotic and abiotic-stress signaling, but functional analysis of its multiple receptors has largely focused on the ubiquitously expressed BRASSINOSTEROID INSENSITIVE 1 (BR1). The vascular-expressed brassinosteroid receptor kinase BRI1-LIKE 3 (BRL3) has a specific and essential role in Arabidopsis adaptation to drought and elevated temperature. However, the mechanistic understanding of BRL3 function and regulation is scant. Here, we show the dehydrin ERD14 colocalizes with BRI1 and BRL3 at the plasma membrane and physically interacts with these receptors through the ERD14Lys domain for proper receptor recruitment. erd14 mutants internalize BRL3-GFP and revert drought-resistant BRL3 over-expression phenotypes. BRL3 activation and downstream signaling events, including dephosphorylation of BES1 transcription factor, depend on the chaperone function of ERD14. Our study shows how proper chaperoning by ERD14 regulates BRL3 subcellular localization and activity of the vascular receptor, prompting re-examination of models of brassinosteroid-receptor functional diversification in plant adaptation to abiotic stress from specific cells of the vascular systems.

plant biology↗

bioRxiv2024

TOTEM: A web TOol for Tissue-EnrichMent analysis on gene lists

Analysis of spatiotemporal patterns of gene expression is crucial to decode biological systems responses. High-throughput sequencing allows in-depth transcriptome analyses and experimental designs, providing valuable reference expression atlases. Specifically, testing overrepresentations of tissue-specific genes based on these atlases can provide valuable insights; however, such an approach is not accessible to inexperienced users. Here, we introduce TOTEM (TOol for Tissue-EnrichMent), a web tool designed to calculate enrichment values per tissue by identifying tissue-specific genes from an organ/organism of interest given a user gene list. Results are visually represented, and users gene classified. The utility of TOTEM is manifest when using integrated single cell expression atlases, enabling the study of complicated tissues, with the maximum possible resolution. Its effectiveness is validated by the study of BRL3 role in stress specifically from the vascular tissues. Finally, TOTEMs modular design allows for continual integration of new experiments. TOTEM can be freely accessed at: https://totemwebtool.com.

plant biology↗

bioRxiv2023

Brassinosteroid receptor BRL3 triggers systemic plant adaptation to elevated temperature from the phloem cells

Understanding plant receptor signaling is crucial to mitigate climate change impact on agriculture. BRs bind to membrane receptor-kinase BR-INSENSITIVE 1 (BRI1) in most plant cells that is essential to promote growth and stress responses, while the roles of vascular BRI1-LIKE1 and 3 (BRL3) receptors were considered redundant. While going unnoticed for twenty years, our study unveils that brl3 mutants show conditional phenotypes to climate stress factors such as elevated temperatures, water deprivation and rising CO2 levels. In response to adverse climate conditions such as elevated temperature, BRL3 signaling at the phloem-companion cells can promote growth by activating BRI1-EMS-SUPPRESSOR1 (BES1) effector, hormonal homeostasis, and central carbon metabolism. This study shifts the paradigm for our present understanding of BR signaling and opens innovative strategies to develop climate-smart crops. One sentence summaryPhloem-specific BRL3 receptor pathway controls plant adaption to elevated temperature.

plant biology↗

bioRxiv2020

Precise transcriptional control of cellular quiescence by BRAVO/WOX5 complex in Arabidopsis roots

Root growth and development are essential features for plant survival and the preservation of terrestrial ecosystems. In the Arabidopsis primary root apex, stem-cell specific transcription factors BRAVO and WOX5 co-localize at the Quiescent Center (QC) cells, where they repress cell division so that these cells can act as a reservoir to replenish surrounding stem cells, yet their molecular connection remains unknown. Here, by using empirical evidence and mathematical modeling, we establish the precise regulatory and molecular interactions between BRAVO and WOX5. We found that BRAVO and WOX5 regulate each other besides forming a transcription factor complex in the QC necessary to preserve overall root growth and architecture. Our results unveil the importance of transcriptional regulatory circuits at the quiescent and stem cells to the control of organ initiation and growth of plant tissues.

plant biology↗

bioRxiv2018

MyROOT: A novel method and software for the semi-automatic measurement of plant root length

Root analysis is essential for both academic and agricultural research. Despite the great advances in root phenotyping and imaging however, calculating root length is still performed manually and involves considerable amounts of labor and time. To overcome these limitations, we have developed MyROOT, a novel software for the semi-automatic quantification of root growth of seedlings growing directly in agar plates. Our method automatically determines the scale from the image of the plate, and subsequently measures the root length of the individual plants. To this aim, MyROOT combines a bottom-up root tracking approach with a hypocotyl detection algorithm. At the same time as providing accurate root measurements, MyROOT also significantly minimizes the user intervention required during the process. Using Arabidopsis, we tested MyROOT with seedlings from different growth stages. Upon comparing the data obtained using this software with that of manual root measurements, we found that there are no significant differences (t-test, p-value < 0.05). Thus, MyROOT will be of great aid to the plant science community by permitting high-throughput root length measurements while saving on both labor and time.

plant biology↗