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Caldwell, A. R.

Publications and source records attributed to Caldwell, A. R..

3 recordsLinked to original sources

Agreement between the ventilated capsule and the KuduSmart(R) device for measuring sweating responses to passive heat stress and exercise

The present study assessed agreement between the KuduSmart(R) device and the ventilated capsule (VC) technique for measuring: i) minute-averaged local sweat rate (LSR); ii) sweating onset; iii) thermosensitivity; and iv) steady-state LSR, during passive heat stress and exercise. On two separate occasions for each heat stress intervention, participants were either passively heated by recirculating hot water (49{degrees}C) through a tube-lined garment until rectal temperature increased 1{degrees}C over baseline (n=8), or walked on a treadmill for 60 minutes at a fixed rate of heat production (n=9). LSR of the forearm was concurrently measured with a VC and the KuduSmart(R) device secured within [~]2 cm. Mean body temperature was calculated as the weighted average between rectal (80%) and mean skin (20%) temperature. Using a ratio scale Bland-Altman analysis with the VC as the reference, the KuduSmart(R) device demonstrated systematic bias for minute-averaged LSR (1.19 [1.09, 1.28]) and steady-state LSR (1.16 [1.09,1.23]), however no bias for thermosensitivity (1.08 [1.00, 1.16]) or sweating onset (1.00 [1.00, 1.00]). Although poor agreement was observed for minute-averaged LSR (CV = 43.3%), more than 73% of all observations with the KuduSmart(R) device (n=2795) were within an absolute error of < 0.2 mg/cm2/min to the VC. The KuduSmart(R) device demonstrated acceptable agreement for steady-state LSR (CV = 19.5%) and thermosensitivity (CV = 21.7%), and almost perfect agreement for sweating onset (CV = 0.14%). Collectively, the KuduSmart(R) device may be a satisfactory in-field solution for assessing the sweating response to heat stress.

physiology↗

Combined Effects of Standing and Passive Heating on Attenuating Sitting-Induced Vascular Dysfunction

Recent evidence suggests prolonged sitting strains the arteries through angulations that create turbulent blood flow. This turbulent flow reduces flow-mediated dilation (FMD), a key indicator of vascular health. The purpose of this study was to determine if arterial angulations (through sitting or standing), shear stress (through local heating), or a combination of these factors affected vascular function. In addition, we sought examined the impact of biological sex on these responses. MethodsTwenty-six healthy, young (18-40 years old) males (n=13) and females (n=13) participated. Participants completed two experimental trials (2-h sitting and 2-h standing), and, in a randomized fashion, one leg was passively heated. Flow-mediated dilation (FMD) at the superficial femoral artery (SFA), and central and peripheral pulse-wave velocity (PWV) were measured using vascular ultrasound. ResultsThere was a non-significant decrease in FMD (- 1.48%; p = .06) during sitting and the decline in FMD was not different between biological sexes (-1.96% vs -0.93%; p = .49, males and females respectively). Passive heating (1.42%; p < .05) and standing (1.42%; p < .05) both improved FMD in comparison to sitting. However, standing resulted in a significant increase in peripheral PWV (50 cm/s; p < .05). Interestingly, the standing was not well tolerated among female participants with seven participants having to stop their initial trial early due to lightheadedness. ConclusionsBoth interventions appear to be equally effective at mitigating reductions FMD associated with sitting, but standing increased peripheral PWV. In addition, it does not appear that biological sex moderates these physiological responses. New FindingsO_ST_ABSWhat is the central question of this study?C_ST_ABSProlonged sitting can cause acutely vascular dysfunction while interventions such as local heating or standing have been explored they have not been used in combination and the role of biological sex has not been fully explored. What is the main finding and its importance?In this study we demonstrate that either local heating or standing are effective at reducing some of the vascular dysfunction associated with prolonged sitting. Biological sex did not appear appear to play a role in this response. However, standing may also cause some negative effects such as increased arterial stiffness and increase the risk of syncope.

physiology↗

Hingepoints and neural folds reveal conserved features of primary neurulation in the zebrafish forebrain

ABSTRACTPrimary neurulation is the process by which the neural tube, the central nervous system precursor, is formed from the neural plate. Incomplete neural tube closure occurs frequently, yet underlying causes remain poorly understood. Developmental studies in amniotes and amphibians have identified hingepoint and neural fold formation as key morphogenetic events and hallmarks of primary neurulation, the disruption of which causes neural tube defects. In contrast, the mode of neurulation in teleosts such as zebrafish has remained highly debated. Teleosts are thought to have evolved a unique pattern of neurulation, whereby the neural plate infolds in absence of hingepoints and neural folds (NFs), at least in the hindbrain/trunk where it has been studied. We report here on zebrafish forebrain morphogenesis where we identify these morphological landmarks. Our findings reveal a deeper level of conservation of neurulation than previously recognized and establish the zebrafish as a model to understand human neural tube development.Competing Interest StatementThe authors have declared no competing interest.View Full Text

developmental biology↗