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Burkhardt, J.

Publications and source records attributed to Burkhardt, J..

3 recordsLinked to original sources

Aerosol deposition affects water uptake and water loss of beech leaves

The deposition of aerosols on leaves could significantly influence plant-atmosphere-interaction through the formation of very thin aqueous films that allow the transport of liquid water through the stomata. Such films can be formed by deliquescence and dynamic expansion of hygroscopic aerosols ( hydraulic activation of stomata). Two processes that may be associated with stomatal liquid water transport are foliar water uptake (FWU) and the contribution of leaky stomata to minimum epidermal conductance (gmin). We investigated whether ambient aerosols affect FWU and gmin of Fagus sylvatica seedlings. Plants were grown in ventilated greenhouses with ambient air or filtered, almost aerosol-free air. The gmin was determined using leaf drying curves. FWU was investigated gravimetrically and with deuterium- enriched water, starting from different leaf water potentials, by spraying freshly-cut or pre-dried leaves (60 minutes). The presence of aerosols in the environment increased gmin by about 47%, confirming previous measurements in other species. Aerosols also increased FWU measured by deuterium uptake. FWU was higher for freshly-cut leaves than for pre-dried leaves, despite the lower leaf water potential. No gravimetric weight gain could be detected. Both the gmin and FWU results are consistent with bidirectional stomatal transport of liquid water along aerosol-induced pathways. The FWU result could also have been generated by water vapor through reverse transpiration, although the functional contribution of the aerosols would remain unclear. At low leaf water potential, the pathway may dry out and become less functional for FWU, whereas it may still be noticeable as stomatal leakage, given the strong gradient of water potential from the leaf interior to the atmosphere.

plant biology↗

Aerosol deposition increases conductance to water in immobilized stomata closed with abscisic acid or opened with fusicoccin

Hypothesized effects of aerosol deposition on plant water balance have been difficult to establish. This is due to variability between species, stomatal response to the treatment itself, and to environmental effects. Here we attempt a quantitative evaluation with a defined aerosol application, a paired leaf experimental design, and immobilized stomata. Attached leaves of poplar were treated with ammonium nitrate aerosol. After 17 or 20 days for deliquescence to develop an aqueous film, leaves were excised and stomata held closed with abscisic acid or open with fusicoccin. Transpiration and stomatal conductance were measured in a greenhouse with a porometer and leaf health was assessed by fluorescence. Median stomatal conductance was increased significantly, by 60 and 65%, following aerosol loading of 31.3 g cm-2 in ABA- and FC-treated leaves, respectively. Aerosol induced transpiration, probably associated with a liquid film that lines the stomatal pore and not effectively regulated by stomatal closure, may be significant in magnitude. As aerosol deposition is ubiquitous, and its chemical nature may be changing, this factor should be considered in models of transpiration from leaf to canopy scale.

plant biology↗

Plants perceive aerosols as an intensification of atmospheric dryness and react according to their isohydricity

Hygroscopic aerosols deposited to leaves are a local water vapor sink and can affect the water balance of plants by deliquescence and the formation of hydraulic films that penetrate into the stomata. Stomatal responses to aerosols and vapor pressure deficit(VPD) were investigated in two poplar clones grown hydroponically in ventilated greenhouses with and almost without ambient aerosols. With increasing VPD, transpiration increased in ANI, the more anisohydric clone, and decreased in ISO, the more isohydric clone, while aerosols had little effect. In ANI, stomatal conductance (gsw) and photosynthesis (A) decreased slightly with increasing VPD, but significantly with exposure to aerosols. Leaf carbon isotopes confirmed the long-term reduction in stomatal aperture by aerosols. In ISO, gsw and A decreased strongly with increasing VPD. Aerosols had no effect on stomatal conductance in ISO, but increased the minimum leaf conductance and decreased the turgor loss point. In both clones, aerosols reduced stomatal density by >20%, indicating increased water scarcity. Aerosols enhance the transmission of atmospheric dryness to the leaf, with plant responses depending on their isohydricity. Sensitive stomatal closure of isohydric plants is an effective adaptation to atmospheric dryness, but aerosol accumulation mediates a liquid pathway for water loss that undermines stomatal control.

plant biology↗