Biology subjects

Burgess, E.

Publications and source records attributed to Burgess, E..

4 recordsLinked to original sources

bioRxiv2025

Disease modulation by TIV vaccination during secondary pneumococcal infections in influenza-infected mice.

Secondary bacterial infections can significantly worsen the clinical course of influenza virus infections and are a leading cause of morbidity and mortality during seasonal influenza epidemics. Despite being a vaccine-preventable disease, influenza-related complications from secondary bacterial infections are an important cause of death, particularly among the elderly population. Streptococcus pneumoniae (Spn) is the most common agent responsible for influenza-related secondary bacterial infections. Influenza virus vaccination serves as an effective prophylactic strategy for preventing influenza and reducing the burden of influenza-associated pathology, including secondary bacterial infection. However, whether the protective effects of influenza virus vaccination differ in the context of a secondary Spn infection at the level of the host response remains poorly characterized. Here, we present a preclinical mouse model to examine the impact of influenza vaccination in scenarios involving single infections with influenza A virus H1N1 (NC99) or Spn serotype 1; simultaneous infection with both NC99 and Spn (coinfection), or NC99 infection followed by Spn infection seven days later (superinfection). A single dose of trivalent inactivated Influenza vaccine (TIV) is able to decrease infection lethality in both secondary bacterial infection scenarios. Protection is associated with reduction in both viral and bacterial titers, decreased production of pro-inflammatory cytokines, protection of alveolar macrophages, prevention of exacerbated lung neutrophil recruitment, modulation of neutrophil activation status and induction of lung eosinophil recruitment and activation. These findings underscore the importance of influenza vaccination in modulating disease progression and preventing morbidity and mortality associated with secondary bacterial infections.

microbiology↗

bioRxiv2025

A combination TLR7/8 and RIG-I agonist adjuvant reverts asthmatic allergic sensitization and prevents aggravated influenza infection in OVA-sensitized mice.

Allergen-specific immunotherapy (AIT) is the only disease-modifying treatment currently available to treat allergy. However, it has limitations, as most allergens are poorly immunogenic, resulting in an AIT process that can take years. Therefore, adjuvant selection becomes critical to achieve a more efficacious therapy. Our group has developed and tested an amphiphilic TLR7/8 agonist (IMDQ) and a RIG-I agonist (SDI) that used alone, or in combination, have demonstrated strong adjuvant activity for influenza and SARS-CoV-2 vaccines in preclinical models. Here we describe the effect of these adjuvants in the sensitization of preclinical models with the ovalbumin (OVA) asthmatic allergic model via an in-depth humoral and cellular immune profiling. We assess their immune skewing and tolerance inducing capacities in previously sensitized preclinical models with different genetic backgrounds (C57BL/6 vs. BALB/c mice). Moreover, we evaluate their effect in an unrelated antigenic challenge with influenza. Finally, we investigate the role of IgG subclasses and T-cell subpopulations in the protection against OVA challenge conferred by the combination of IMDQ and SDI. We demonstrate that OVA-immunization in combination with IMDQ+SDI prevents allergic sensitization via the induction of a balanced Type 1/Type 2 response. Furthermore, it can revert the allergic phenotype in mice previously sensitized with OVA-Alum, through reducing lung eosinophilia, as well as IL-4 and IL-5 production. However, this was dependent on genetic background. IMDQ+SDI sensitization also led to reduced morbidity of a secondary influenza challenge in OVA-sensitized mice. Finally, we demonstrated that IgG2c, by itself, cannot protect from allergic sensitization and that both CD4+ and CD8+ T-cells are needed for IMDQ+SDI prevention of eosinophil recruitment and activation upon intranasal OVA-challenge.

immunology↗

bioRxiv2025

Claustrophilic oviposition: oviposition performance depends on container size in a novel forced oviposition method for Culex quinquefasciatus and Aedes aegypti

Mosquito vector control research relies heavily on the use of live organisms, with wild populations playing a critical role in surveillance, field product validation, and strengthening the overall efficacy and accuracy of studies. However, too often wild mosquito rearing and colony establishment in laboratory settings is consistently challenging. Here we present a modified version of a forced oviposition method for use against two different mosquito species, Aedes aegypti and Culex quinquefasciatus. The efficacy of the technique was tested with both laboratory and wild-caught strains placed in oviposition tubes consisting of a 1.5 mL tube containing moistened cotton and a strip of germination paper. To determine if size had an impact on oviposition rate, an additional test was conducted using four different size oviposition tubes; 1.5, 5, 15, and 50 mL. Overall, the forced oviposition method was highly effective, successfully generating eggs from lab reared and field collected strains. The method was more effective with Ae. aegypti (>80% females oviposited) compared to Cx. quinquefasciatus (50-60%) in both strains. Culex quinquefasciatus holding time was longer (3-7 days) compared to Ae. aegypti females, which oviposited within 24 hr of being transferred into tubes. Intact versus broken egg rafts affected egg hatch rate in laboratory Cx. quinquefasciatus strains but had less impact on the wild strain. Additionally, both Ae. aegypti and Cx. quinquefasciatus displayed a claustrophilic oviposition behavior with a higher percentage of females ovipositing in the 1.5 and 5 mL tubes. This study demonstrates that the forced oviposition method can be easily adapted to other mosquito vector species and effective in producing F1 progeny needed for critical vector research. Author SummaryMosquito vector control programs rely heavily on data and information generated from research and phenotypic insecticide resistance monitoring, which require the use of live mosquitoes, both from laboratory colonies and field caught populations. Here we tested a modified version of a force oviposition technique originally designed for establishing wild Anopheles spp. colonies, on two key mosquito vectors, Culex quinqufasciatus and Aedes aegypti. We find that, despite their distinct oviposition preferences and behaviors, both species of mosquitoes generated adequate numbers of F1 eggs needed to start either laboratory or wild colonies. A key advantage to this method is its ease of use, only requiring minimal numbers of female mosquitoes and inexpensive, easily accessible materials commonly found in most laboratories and mosquito control districts. Further application of the forced oviposition method has important implications for enhancing our capacity to conduct a range of vector research with various key mosquito species. This could include key vector research projects such as, determining single female sex ratios, producing genetic isolines by controlling specific genetic backgrounds, and conducting heritability studies focused on selective mating.

zoology↗

bioRxiv2023

Expression, activity, and consequences of biochemical inhibition of α- and β-glucosidases in different life stages of Culex quinquefasciatus

Mosquitoes utilize a plethora of digestive enzymes to meet the challenge of both requisite blood and sugar meals that enable them to survive and reproduce. Sugar meals, typically derived from plant sources, are critical to maintain energy in both male and female mosquitoes, whereas blood meals are taken only by females to complete oogenesis. Enzymes involved in sugar digestion have been the subject of study for decades but have been limited to a relatively narrow range of mosquito species. The southern house mosquito, Culex quinquefasciatus, is of public health importance and seldom considered in these types of studies outside of topics related to Bacillus sphaericus, a biocontrol agent that requires interaction with a specific gut-associated -glucosidase. Here we sought to describe the nature of -glucosidases and the unexplored {beta}-glucosidases that may aid Cx. quinquefasciatus larvae in acquiring nutrients from cellulosic sources in their aquatic environments. Consistent with our hypothesis, we found both - and {beta}-glucosidase activity in larvae. Interestingly, {beta}-glucosidase activity all but disappeared at the pupal stage and remained low in adults, while -glucosidase activity remained in the pupal stage and then exceeded larval activity by approximately 1.5-fold. Expression of the putative - and {beta}-glucosidase genes chosen did not generally follow the trends seen in enzyme activities. When the -glucosidase inhibitor acarbose was administered to adults, mortality was seen especially in males but also in females after two days of exposure and key energetic storage molecules, glycogen and lipids, were significantly lower than controls. In contrast, administering the {beta}-glucosidase inhibitor conduritol {beta}-epoxide to larvae did not produce mortality even at the highest soluble concentration. Here we provide insights into the importance of - and {beta}-glucosidases on the survival of Cx. quinquefasciatus in their three mobile life stages.

zoology↗