Biology subjects

Brembs, B.

Publications and source records attributed to Brembs, B..

2 recordsLinked to original sources

bioRxiv2019

MARGO (Massively Automated Real-time GUI for Object-tracking), a platform for high-throughput ethology

Fast object tracking in real time allows convenient tracking of very large numbers of animals and closed-loop experiments that control stimuli for multiple animals in parallel. We developed MARGO, a real-time animal tracking suite for custom behavioral experiments. We demonstrated that MARGO can rapidly and accurately track large numbers of animals in parallel over very long timescales. We incorporated control of peripheral hardware, and implemented a flexible software architecture for defining new experimental routines. These features enable closed-loop delivery of stimuli to many individuals simultaneously. We highlight MARGOs ability to coordinate tracking and hardware control with two custom behavioral assays (measuring phototaxis and optomotor response) and one optogenetic operant conditioning assay. There are currently several open source animal trackers. MARGOs strengths are 1) robustness, 2) high throughput, 3) flexible control of hardware and 4) real-time closed-loop control of sensory and optogenetic stimuli, all of which are optimized for large-scale experimentation.

neuroscience↗

bioRxiv2018

Differential dominance of an allele of the Drosophila tbetah gene defies standard genetic techniques

The biogenic amine octopamine (OA) and its precursor tyramine (TA) are involved in controlling a plethora of different physiological and behavioral processes. The tyramine-{beta}-hydroxylase (t{beta}h) gene encodes the enzyme catalyzing the last synthesis step from TA to OA. Here, we report differential dominance (from recessive to overdominant) of the putative null t{beta}hnM18 allele in two behavioral measures in Buridans paradigm (walking speed and stripe deviation) and a proboscis extension assay in the fruit fly Drosophila melanogaster. The behavioral analysis of transgenic t{beta}h expression experiments in mutant and wild type flies as well as of OA- and TA-receptor mutants revealed a complex interaction of both aminergic systems. Our analysis suggests that the different neuronal networks responsible for the three phenotypes show differential sensitivity to t{beta}h gene expression levels. The evidence suggests that this sensitivity is brought about by a TA/OA opponent system modulating the involved neuronal circuits. This conclusion entails important implications for standard transgenic techniques, commonly used in functional genetics.

genetics↗