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Braunschweig, U.

Publications and source records attributed to Braunschweig, U..

2 recordsLinked to original sources

Regulation of transcription termination by FUS and TDP-43

The carboxy-terminal domain (CTD) of the RNA polymerase II (RNAPII) subunit POLR2A is a platform for modifications specifying the recruitment of factors that regulate transcription, mRNA processing, and chromatin remodelling. We previously found that symmetrical dimethylation (me2s) of a CTD Arginine residue (R1810 in human) causes recruitment of the Tudor domain of SMN, which interacts with Senataxin. SMN is mutated in spinal muscular atrophy (SMA), and Senataxin is sometimes mutated in Amyotrophic Lateral Sclerosis (ALS). R1810me2s and SMN, like Senataxin, are important for resolving R-loops (DNA:RNA hybrids) at transcription terminators. FUS and TDP-43 (TARDBP) are DNA/RNA binding proteins that are sometimes mutated in ALS and FTD (Frontotemporal dementia). Here we show that TDP-43 and, to some extent, FUS are recruited by the R1810me2s-SMN pathway. Defects in FUS and TDP-43 recruitment influence RNAPII termination and R-loop accumulation, leading to elevated DNA damage at terminators that may contribute to neurodegenerative disorders like ALS and FTD.

molecular biology

Ubiquitylated H2A.Z nucleosomes are associated with nuclear architectural proteins and global transcriptional silencing

H2A.Z monoubiquitylation has been linked to transcriptional repression, but the molecular details involved remain unclear. To address this, we developed a unique ubiquitylation-dependent auto-biotinylation approach, named H2A.Z-UAB, to purify ubiquitylated H2A.Z (H2A.Zub) mononucleosomes for biochemical and genome-wide analyses. We found that H2A.Zub nucleosomes are enriched for the repressive histone post-translational modification H3K27me3 but depleted of H3K4 methylation and other active transcription-associated modifications. ChIP-seq analyses revealed that H2A.Zub-nucleosomes associate with repressive chromatin states and are mostly enriched at genes with low or no expression. Consistent with the biochemical analyses, H2A.Zub ChIP-seq peaks align with regions depleted of H3K4 methylation, H3K27 acetylation, but enriched for H3K27me3. We further observed a strong correlation between high levels of H2A.Zub and DNA methylation, particularly at methylated CpG-rich islands. Finally, we found that a significant proportion of H2A.Zub peaks correspond to homopurine/homopyrimidine sequences that are generally linked to silenced genes and repressive chromatin. Collectively, these findings suggest that H2A.Z ubiquitylation plays an overarching role in global transcriptional silencing through its association with multiple types of repressive mechanisms. GRAPHICAL ABSTRACT O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=88 SRC="FIGDIR/small/759852v3_ufig1.gif" ALT="Figure 1"> View larger version (11K): org.highwire.dtl.DTLVardef@71804eorg.highwire.dtl.DTLVardef@19eb987org.highwire.dtl.DTLVardef@1a67e15org.highwire.dtl.DTLVardef@19fc5c2_HPS_FORMAT_FIGEXP M_FIG C_FIG

biochemistry