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Brasseur, T.

Publications and source records attributed to Brasseur, T..

2 recordsLinked to original sources

Overlapping flower and pollen production in two imperiled pitcher plants raises conservation challenges

Balancing sexual reproduction with vegetative growth poses challenges for many plants. Additionally, reproductive effort, such as production of pollen or flowers, is not always correlated with numbers of viable and germinable seeds. Energetic tradeoffs like these can be particularly fraught for imperiled species and might be especially complicated in landscapes with a possibility of interspecific hybridization. In this multi-year study, we track the reproductive effort, flowering phenology, and reproductive output of two interfertile Sarracenia (pitcher plants). We show that reproductive effort varies among years and sites, and that production of pollen and flowers does not always result in higher seed output. We demonstrate that phenological overlap in the timing of flower production and pollen viability could enable hybridization, and that the more imperiled taxon produces fewer seeds per flower. Results have implications for conservation of these pitcher plants, management of hybridization in these and other systems, and general principles of reproductive allocation.

ecology↗

Enhancing cryopreservation of ex vivo 3D tumor models using vitrification strategies

Microdissected tumor tissue explants (MDTs) are promising ex vivo models for oncology research but remain limited by poor preservation and the rapid loss of viability following resection. Here, we systematically optimized MDT cryopreservation using prostate-derived 49F and ovarian TOV112D tumor models. Before cryopreservation, we evaluated the effects of antioxidant supplementation, pre-cooling, and a short recovery period. MDTs were then preserved by either conventional slow-freezing or vitrification using ultra-rapid cooling. Following thawing, tissue morphology, apoptosis, and proliferative capacity were assessed relative to fresh controls. Vitrification improved morphological preservation and reduced apoptosis compared with slow-freezing, although recovery of proliferation differed between tumor models. Antioxidant supplementation enhanced post-thaw proliferation at optimal concentrations but induced toxicity at higher doses. Pre-cooling and a short pre-cryopreservation recovery period further improved post-thaw outcomes. Combining these parameters produced an optimized vitrification protocol that preserved up to 98% of the proliferative capacity of 49F MDTs and 69% of that of TOV112D MDTs relative to fresh controls. These findings establish optimized vitrification as a reproducible, high-yield strategy for preserving MDTs for downstream ex vivo oncology applications.

cancer biology↗