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Bosch, M.

Publications and source records attributed to Bosch, M..

2 recordsLinked to original sources

Spatiotemporal patterns of urbanization in three Swiss urban agglomerations: insights from landscape metrics, growth modes and fractal analysis

Urbanization is currently a global phenomenon that has become the most important form of landscape change and is increasingly affecting biodiversity and ecosystem functions. In order to evaluate the impacts of urbanization and inform urban planning, it is important to understand the spatiotemporal patterns of land use change associated to urbanization. This paper exploits three different frameworks, namely landscape metrics, urban growth modes and fractal analysis to characterize the spatiotemporal patterns of urbanization of the Swiss urban agglomerations of Zurich, Bern and Lausanne. The land use inventory provided by the Swiss Federal Statistical Office was used to assemble four temporal snapshots from 1980 to 2016 at the extent of the urban agglomerations. The time series of landscape metrics generally supports the diffusion and coalescence model of urban growth, with Zurich exhibiting most characteristics of coalescence while Bern and Lausanne seem to be at the transition between diffusion and coalescence. Nevertheless, the analysis of the urban growth modes suggest that leapfrog development occurs at all periods, which contributes to an increasing fragmentation of natural patches and maintains the fractal configuration of the landscape. The discussion reviews potential explanations for the observed landscape changes, and concludes with some planning implications.

ecology

Reversible silencing of endogenous receptors in intact brain tissue using two-photon pharmacology

The physiological activity of proteins is often studied with loss-of-function genetic approaches, but the corresponding phenotypes develop slowly and can be confounding. Photopharmacology allows direct, fast and reversible control of endogenous protein activity, with spatiotemporal resolution set by the illumination method. Here, we combine a photoswitchable allosteric modulator (alloswitch) and two-photon excitation (2PE) using pulsed near-infrared lasers to reversibly silence mGlu5 receptor activity in intact brain tissue. Endogenous receptors can be photoactivated in neurons and astrocytes with pharmacological selectivity and with an axial resolution between 5 and 10 m. Thus, two-photon pharmacology (2PP) using alloswitch allows investigating mGlu5-dependent processes in wild type animals, including synaptic formation and plasticity, and signaling pathways from intracellular organelles.

pharmacology and toxicology