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Boonyaves, K.

Publications and source records attributed to Boonyaves, K..

2 recordsLinked to original sources

Image-based leaf SPAD value and chlorophyll measurement using a mobile phone: enabling accessible and sustainable crop management

AO_SCPLOWBSTRACTC_SCPLOWThis study evaluates a practical, low-cost solution for image-based leaf SPAD (Soil and Plant Analysis Development) value and chlorophyll content monitoring using a mobile phone. We compare laboratory assay and SPAD-502+ measurements with image-based estimates from a mobile phone app (PhotoFolia). Performance is tested for four commercial rice varieties grown in Thailand. Results show that the image-based method can predict SPAD values within {+/-} 1.2 units Mean Absolute Error (MAE) and- chlorophyll concentrations within 7.2% Mean Absolute Percentage Error (MAPE) of laboratory results. Achieving a SPAD value error close to the industry standard of {+/-}1 unit and a relative error of less than 10% in chlorophyll concentration estimation (compared to a laboratory method) demonstrates that an image-based approach using standard mobile phones can serve as an accessible, low-cost tool for on-farm chlorophyll monitoring, without the need for specialised equipment. Key Points / HighlightsNovel low-cost approach for chlorophyll assay and SPAD-value measurement using standard mobile phone. Achieves accuracy comparable to commercial tools. Eliminates need for specialised sensors or laboratory equipment. ImpactThis study demonstrates that mobile phone-based image analysis can accurately estimate leaf SPAD and chlorophyll levels in rice under ambient lighting conditions, offering a low-cost, accessible tool for monitoring plant health.

plant biology↗

Evolutionarily Conserved Heat-Induced Chromatin Dynamics Drive Heat Stress Responses in Plants

Eukaryotic organisms remodel chromatin landscapes to regulate gene expression in response to environmental stress. In plants, heat stress (HS) induces widespread chromatin changes, yet the role of heat-responsive Heat Shock Transcription Factors (HSFs) in chromatin remodeling and their evolutionary conservation remains unclear. Using chromatin accessibility profiling and transcriptomics in Marchantia polymorpha hsf mutants, we identify HSFA1 as a key determinant in positioning cis-regulatory elements (CREs) for HS-induced gene activation, a mechanism conserved across land plants, mice, and human cells. By integrating gene regulatory network modeling, we identify parallel transcription factor subnetworks, with MpWRKY10 and MpABI5B acting as indirect regulators of HS responses via phenylpropanoid pathways and general stress signaling. We further explore crosstalk between HS and abscisic acid (ABA) signaling, showing that while ABA modulates gene expression in an HSFA1-dependent manner, it does not induce broad chromatin remodeling, positioning it as a downstream regulator rather than a primary determinant of chromatin dynamics. To extend these insights, we develop a cross-species and cross-condition machine learning framework that accurately predicts chromatin accessibility and gene expression, demonstrating a conserved regulatory logic of stress-responsive chromatin and transcription dynamics. Our findings provide a conceptual framework for understanding how TFs coordinate chromatin architecture to drive stress adaptation in plants and potentially other eukaryotes.

plant biology↗