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Bogers, J.

Publications and source records attributed to Bogers, J..

2 recordsLinked to original sources
bioRxiv2025

Application of nucleoside analogue labelling to study the cell cycle of xenografted PDAC cell lines in the chorioallantoic membrane model.

The chorioallantoic membrane (CAM) model is an underutilised alternative model for the research into tumour xenografts. We demonstrate that in the pancreatic cancer cell lines BxPC-3 and AsPC-1, nucleoside labelling with 5-ethynyl-2'-deoxyuridine (EdU) can be multiplexed with other human cell cycle markers, (e.g., cyclin B1 and Ki-67), particularly when combined with digital image analysis, allowing human versus chicken cell classification. Furthermore, starting from the embryonic day of development 14 (ED14), we observe embryonic cells exhibiting substantial apparent cytoplasmic accumulation of synthetic nucleosides. This was observed for (F-ara-)EdU as well as for the halogenated analogues BrdU and IdU. Characterisation of these cells suggests that they are non-proliferating and possess granules and can be found in the embryonic liver of (non-)grafted embryos, as well as in the CAM itself and within xenograft sections. Importantly, these cells are unlikely to represent activated professional antigen presenting cells as they lack MHC II expression, nor do they express CD41/CD61, ruling out thrombocyte origin. Together, our findings demonstrate that the CAM model constitutes a valuable platform for studying tumour cell cycle dynamics in vivo. However, nucleoside labelling applications beyond ED14 may be confounded by a previously unidentified phenomenon, namely cytoplasmic nucleoside accumulation in certain chicken cells.

cell biology↗
bioRxiv2018

Prevalence and genotype-specific distribution of Human papillomavirus in Burundi according to HIV status and urban or rural residence and its implications for control

BackgroundHuman papillomaviruses are the most important causative agents for invasive cervical cancer development. HPV type-specific vaccination and HPV cervical cancer screening methods are being widely recommended to control the disease but the epidemiology of the circulating HPV types may vary locally. The circulating HPV-strains have never been assessed in Burundi. This study determined the prevalence and genotype-specific distribution of HPV in four different strata in Burundi: HIV-infected or non-infected and women living in rural or urban areas. Implications for HPV diagnosis and vaccine implementation was discussed.\n\nMethodsFour cross-sectional surveys were conducted in Burundi (2013 in a rural area and 2016 in urban area) among rural and urban HIV-infected and uninfected women. Cytology and HPV genotyping was performed to screen women for cervical cancer lesions. Risk factors for HPV infection and cervical cancer lesions were determined using logistic regression model.\n\nResultsHPV prevalence was very high in urban area with significant differences between HIV-positive and negative women (p<0.0001). In fact, 45.7% of HIV-positive participants were infected with any HPV type and all were infected with at least one HR/pHR-HPV type. Among the HIV-negative participants, 13.4% were HPV-infected, of whom, only 4 women (2.7%) were infected with HR/pHR-HPV types. In rural, HPV infection did not significantly differ between HIV-positive and negative women (30.0% and 31.3% respectively; p=0.80).\n\nIn urban, multiple infections with HR/pHR-HPV types were detected in 13.9% and 2.7% among HIV-positive and negative women respectively (p<0.0001), whereas in rural, multiple infections with HR/pHR-HPV types were detected in 4.7% and 3.3% of HIV positive and negative women respectively (p=0.56).\n\nThe most prevalent HR/pHR-HPV types in HIV-positive urban women were HPV 52, 51 and 56. In the HIV-negative urban women, the most prevalent HR/pHR-HPV types were HPV 66, 67 and 18. In HIV-positive rural women, the most prevalent HR/pHR-HPV types were HPV 66, 16 and 18. In the HIV-negative rural women, the most prevalent HR/pHR-HPV types were HPV 16, 66 and 18. Independent risk factors associated with cervical lesions were HPV and HIV infections.\n\nConclusionsThere is a high burden of HR/pHR-HPV infections, in particular among HIV-infected urban women. The study points out the need to introduce a comprehensive cervical cancer control program adapted to the context. This study shows that the nonavalent vaccine covers most of the HR/pHR-HPV infections in rural and urban areas among HIV-infected and uninfected women.

epidemiology↗