Biology subjects

Bisgaard, M.

Publications and source records attributed to Bisgaard, M..

3 recordsLinked to original sources

bioRxiv2025

Foot perfusion. Insights from an anatomically detailed arterial network model

Peripheral artery disease currently affects over 202 million people worldwide. The ankle-brachial index is one of the most used measurements to assess a reduction in blood flow to the foot, but it is not able to characterise tissue perfusion. Information on tissue perfusion can be obtained, among others, through an MRI scan, which is time-consuming and can be painful if induction of ischemia is warranted for the scan. As an alternative, we model foot perfusion during a cuff-induced ischaemia test to characterise how occlusions in foot arteries affect perfusion in foot regions. Simulations are not patient-specific at this stage, and are conducted on a 1D arterial network model which includes 154 foot and calf arterial segments, providing a realistic description of the topology of the foot arterial vasculature. A baseline model characterizes perfusion in angiosomes under healthy conditions, which is then modified to reflect 42 pathological scenarios by introducing occlusions and different levels of collateral impairment. Results show a marked influence of collateral impairment on angiosome perfusion under the condition of a single-artery occlusion, highlighting the role of blood redistribution. If two feeding arteries are occluded, perfusion markedly decreases at all collateral impairment levels due to the severe reduction in incoming blood flow. These results provide a bridge between the angiosome-targeted and "best-vessel" strategies for revascularization, showing that both can be correct depending on collateral sufficiency. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=77 SRC="FIGDIR/small/692561v1_ufig1.gif" ALT="Figure 1"> View larger version (22K): org.highwire.dtl.DTLVardef@10d4da5org.highwire.dtl.DTLVardef@649775org.highwire.dtl.DTLVardef@13b8e4org.highwire.dtl.DTLVardef@cfbb3e_HPS_FORMAT_FIGEXP M_FIG C_FIG HighlightsO_LIWe propose a computational model of foot perfusion during a cuff-induced ischaemia test that allows the assessment of perfusion in each angiosome of the foot C_LIO_LIWe study how occlusions in the main feeding arteries of the foot impact tissue perfusion C_LIO_LIWe highlight the role of collateralization if adequate inflow is maintained C_LIO_LIResults show that both angiosome-targeted and "best-vessel" strategies for revascularization can be correct depending on arch patency and collateral sufficiency C_LI

bioengineering↗

bioRxiv2025

Shaping a Collaborative, Sustainable, Accessible, and Reproducible Future for Computational Modeling

The o2S2PARC platform is an open-source, extensible, and scalable cloud-based platform developed in the context of the U.S. National Institutes of Health SPARC program to support collaborative, sustainable, FAIR (findable, accessible, interoperable, reusable) and reproducible computational modeling and analysis. This publication presents the main features of o2S2PARC, its underlying approaches and philosophy, innovative aspects of the developed technologies, while also drawing attention to its rapid adoption. The paper showcases a variety of applications and use cases enabled by the platform. These include hybrid electromagnetic-electrophysiology simulations of neural interfaces, personalized brain and spinal cord stimulation planning, in silico device safety assessments, the training and application of AI systems (e.g., for model-predictive control and medical image segmentation), hybridized surrogate modeling and multi-objective optimization in high-dimensional parameter spaces, sensitive and unbiased validation of measurement devices, and interactive data analysis as paper supplements.

neuroscience↗

bioRxiv2020

Evaluation of a new probiotic concept for broilers

Probiotics were introduced as a spray directly in the hatcher when chickens started to leave the eggs which potentially could reduce the horizontal transmission and colonization with pathogenic bacteria. The single introduction of probiotics could limit the cost compared to multiple introductions with feed and/or water. A mixture of five probiotic strains belonging to Escherichia coli, Enterococcus faecalis, Lactobacillus agilis and Lactobacillus rhamnosus was tested with two independent flocks of broilers (Ross 308). For each experiment, a comparison was made to an untreated control flock on the same farm. At day 14 of production the probiotic strains were re-isolated from ileum of euthanized chickens. The first week mortality was slightly increased in the probiotic flock (0.42%) compared to the control (0.35%) in experiment 1, however, it was higher in the control flock (1.45%) compared to the probiotic flock (1.12%) in experiment 2. The average weight of chickens that could be slaughtered for consumption was increased by 3.5% in the probiotic flocks compared to the control flocks, resulting in a 1.9% higher total weight of slaughtered chickens in the probiotics treated flocks compared to the control as a mean of the two experiments. The number of condemned animals was within the normal range for the production system and could not directly be related to effects of probiotics. Although one probiotic strain of E. coli was isolated from dead animals, the probiotics did not affect the proportion of chickens which died due to E. coli during the first week compared to the control. Primary audienceplant managers, veterinarians, nutritionists

microbiology↗