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Bhattacharyya, P.

Publications and source records attributed to Bhattacharyya, P..

4 recordsLinked to original sources

Alternatives to Friction Coefficient: Fine Touch Perception Relies on Frictional Instabilities

Fine touch perception is often correlated to material properties and friction coefficients, but the inherent variability of human motion has led to low correlations and contradictory findings. Instead, we hypothesized that humans use frictional instabilities to discriminate between objects. Here, we constructed a set of coated surfaces with minimal physical differences, but due to differences in surface chemistry, generated different types of instabilities depending on how quickly a finger is slid and pressed during sliding. In one experiment, we used a mechanical mock finger to quantify and classify differences in instability formation from different coated surfaces. In a second experiment, participants perform a discrimination task using the same coated surfaces. Using the data from these two experiments, we found that human discrimination response times were faster with surfaces where the mock finger produced more stiction spikes and discrimination accuracy was higher where the mock finger produced more steady sliding. Conversely, traditional metrics like surface roughness or average friction coefficient did not relate to tactile discriminability. In fact, the typical method of averaging friction coefficients led to a spurious correlation which erroneously suggests that distinct objects should feel identical and identical objects should feel distinct--similar to findings by others. Friction instabilities may offer a more predictive and tractable framework of fine touch perception than friction coefficients, which would accelerate the design of tactile interfaces.

neuroscience↗

A portable electrochemical DNA sensor for sensitive and tunable detection of piconewton-scale cellular forces

Cell-generated forces are a key player in cell biology, especially during cellular shape formation, migration, cancer development, and immune response. A new type of label-free smartphone-based electrochemical DNA sensor is developed here for cellular force measurement. When cells apply tension forces to the DNA sensors, the rapid rupture of DNA duplexes allows multiple redox reporters to reach the electrode and generate highly sensitive electrochemical signals. The sensitivity of these portable sensors can be further enhanced by incorporating a CRISPR-Cas12a system. Meanwhile, the threshold force values of these DNA-based sensors can be rationally tuned based on the force application geometries and also DNA intercalating agents. Overall, these highly sensitive, portable, cost-efficient, and easy-to-use electrochemical sensors can be powerful tools for detecting different cell-generated molecular forces. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=139 SRC="FIGDIR/small/586508v1_ufig1.gif" ALT="Figure 1"> View larger version (35K): org.highwire.dtl.DTLVardef@e9bb33org.highwire.dtl.DTLVardef@10114d0org.highwire.dtl.DTLVardef@1fd925dorg.highwire.dtl.DTLVardef@942a20_HPS_FORMAT_FIGEXP M_FIG C_FIG

biophysics↗

Electrochemical DNA-based sensors for measuring cell-generated forces

Mechanical forces play an important role in cellular communication and signaling. We developed in this study novel electrochemical DNA-based force sensors for measuring cell-generated adhesion forces. Two types of DNA probes, i.e., tension gauge tether and DNA hairpin, were constructed on the surface of a smartphone-based electrochemical device to detect piconewton-scale cellular forces at tunable levels. Upon experiencing cellular tension, the unfolding of DNA probes induces the separation of redox reporters from the surface of the electrode, which results in detectable electrochemical signals. Using integrin-mediated cell adhesion as an example, our results indicated that these electrochemical sensors can be used for highly sensitive, robust, simple, and portable measurement of cell-generated forces.

biophysics↗

Ethanol's action at BK channels accelerates the transition from moderate to excessive alcohol consumption

Large conductance potassium (BK) channels are among the most sensitive molecular targets of ethanol and genetic variations in the channel-forming subunit have been nominally associated with alcohol use disorders. However, whether the direct action of ethanol at BK influences the motivation to drink alcohol remains to be determined. In the present study, we sought to investigate the behavioral relevance of this molecular interaction by introducing in the mouse genome a point mutation known to render BK channels insensitive to ethanol while preserving their physiological function. The BK K361N substitution prevented ethanol from reducing spike threshold in medial habenula neurons. However, it did not alter acute responses to ethanol in vivo, including ataxia, sedation, hypothermia, analgesia, and conditioned place preference. Furthermore, the mutation did not have reproducible effects on alcohol consumption in limited, continuous, or intermittent access home cage two-bottle choice paradigms conducted in both males and females. Notably, in contrast to previous observations made in mice missing BK channel auxiliary {beta} subunits, the BK K361N substitution had no significant impact on ethanol intake escalation induced by chronic intermittent alcohol vapor inhalation. It also did not affect the metabolic and locomotor consequences of chronic alcohol exposure. Altogether, these data suggest that the direct interaction of ethanol with BK does not mediate the alcohol-related phenotypes examined here in mice.

neuroscience↗