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Bertrand, V.

Publications and source records attributed to Bertrand, V..

2 recordsLinked to original sources

Establishment of Wnt ligand-receptor organization and cell polarity in the C. elegans embryo

Different signaling mechanisms concur to ensure robust tissue patterning and cell fate instruction during animal development. Most of these mechanisms rely on signaling proteins that are produced, transported and detected. The spatiotemporal dynamics of signaling molecules is largely unknown, yet it determines signal activitys range and time frame. Here, we use the Caenorhabditis elegans embryo to study how Wnt ligands, an evolutionarily conserved family of signaling proteins, dynamically organize to establish cell polarity in a developing tissue. We identify how locally produced Wnt ligands spread to transmit information to distant target cells. With quantitative live imaging, we show that the Wnt ligands diffuse extracellularly through the embryo over a timescale shorter than the cell cycle. We extract diffusion coefficients of Wnt ligands and their receptor Frizzled (Fz) and characterize their co-localization. Integrating our different measurements and observations in a simple computational framework, we show how fast diffusion in the embryo can polarize target cells. Our results support diffusion-based long-range Wnt signaling, which is consistent with the dynamics of developing processes.

developmental biology↗

Imaging of native transcription and transcriptional dynamics in vivo using a tagged Argonaute protein

AO_SCPLOWBSTRACTC_SCPLOWA flexible method to image unmodified transcripts and transcription in vivo would be a valuable tool to understand the regulation and dynamics of transcription. Here, we present a novel approach to follow native transcription, with fluorescence microscopy, in live C. elegans. By using the fluorescently tagged Argonaute protein NRDE-3, programmed by exposure to defined dsRNA to bind to nascent transcripts of the gene of interest, we demonstrate transcript labelling of multiple genes, at the transcription site and in the cytoplasm. This flexible approach does not require genetic manipulation, and can be easily scaled up by relying on whole-genome dsRNA libraries. We apply this method to image the transcriptional dynamics of two transcription factors: ttx-3 (a LHX2/9 orthologue) in embryos, and hlh-1 (a MyoD orthologue) in larvae, respectively involved in neuronal and muscle development.

molecular biology↗