Alzheimer’s Amyloid-Beta Intermediates Generated by Polymer-Nanodiscs
Polymethacrylate-copolymer (PMA) encased lipid-nanodiscs (~10 nm) and macro-nanodiscs (>15 nm) are used to study A{beta}1-40 aggregation. We demonstrate that PMA-nanodiscs form a ternary association with A{beta} and regulate its aggregation kinetics by trapping intermediates. Results demonstrating reduced neurotoxicity of nanodisc-bound A{beta} oligomers are also reported.
biophysics↗