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Bapat, P.

Publications and source records attributed to Bapat, P..

3 recordsLinked to original sources

Understudied and underprotected: biodiversity and conservation challenges in the Konkan region of Maharashtra

In biodiversity hotspots, such as the Western Ghats, periodic synthesis of existing ecological research can help identify knowledge gaps and address critical threats to biodiversity. The Maharashtra part of the Konkan region, situated between the Sahyadri foothills and the west coast, with diverse tropical vegetation, harbours unique open ecosystems, such as lateritic plateaus, and supports a multitude of threatened species, including hornbills and tigers. However, the region remains relatively understudied and has not received adequate protection through state or national conservation policies and laws. The area is undergoing rapid human-driven land-use change. These activities can impact the regions biodiversity, necessitating an effort to identify knowledge gaps and critical threats. Through a combination of a literature review and focus group discussions with 44 participants from various institutions and non-governmental organisations, we synthesised existing published information on the Konkan region of Maharashtra and identified key research gaps and conservation challenges. Our review of 138 studies found that while agroforestry and human-wildlife interactions have received some research attention, the effects of climate change on the regions biodiversity remain poorly understood. Focus group discussions highlighted major threats, including land-use changes due to expanding monoculture plantations, clear-felling of forests, forest fires, environmental pollution, and rapid infrastructure development, which are leading to habitat loss and fragmentation. Some of these concerns were validated by publicly available regional data, which revealed a 30% increase in roads, a 14% expansion of cashew plantations, and associated forest loss. This synthesis offers valuable insights for government and non-governmental organisations to inform future research and conservation efforts in the region.

ecology↗

Medulloblastoma-associated DDX3X mutants are oncogenic having a defect in translation-promoting activity but functional in stress granule formation and interferon signaling

DDX3X, a DEAD box-containing RNA helicase, is known to play diverse roles in RNA metabolism, stress response, innate immunity, and cancer. Medulloblastoma is the single most common malignant brain tumor in children. DDX3X is recurrently mutated in the WNT and SHH subgroups of medulloblastoma. CRISPR-Cas9 mediated DDX3X knockout was successful in the HEK293FT cells but generated only non-truncating indels in the medulloblastoma cells suggesting DDX3X is necessary for the viability of the cells. Downregulation of DDX3X expression using shRNA also brought about a considerable reduction in proliferation, clonogenic potential, and anchorage-independent growth of the medulloblastoma cells. Thus, DDX3X expression was found to be essential for the survival, growth, and malignant potential of the medulloblastoma cells consistent with the non-truncating nature of medulloblastoma-associated DDX3X mutations. The medulloblastoma-associated DDX3X mutants were found to be defective in their ability to drive the translation of mRNAs with complex 5-UTR that is dependent on the ATP-dependent helicase activity of DDX3X. These helicase defective DDX3X mutants could restore the expression of interferon signaling genes and malignant potential lost upon DDX3X knockdown in medulloblastoma cells. Their N-terminal domain is intact and was found to be functional in stress granule formation. DDX3X mutants upregulated expression of malignancy-related genes suggesting tumor suppressive role for the helicase activity in the medulloblastoma pathogenesis. Inhibitors of the N-terminal domain of DDX3X which is essential for the viability of medulloblastoma cells could have therapeutic potential in the treatment of WNT and SHH subgroup medulloblastomas.

cancer biology↗

A survey of Rare Disease awareness among healthcare professionals and researchers in India

Rare diseases (RDs) are diseases that occur infrequently and affect a small fraction of the population. Although these diseases individually affect small number of people, together they affect 400 million people globally at any given time. In India, where resources are scarce, healthcare infrastructure and policy framework are focused on mitigating diseases that affect many people. Further, the level of RD awareness among healthcare professionals, researchers, and general public is considerably low. As a result, many cases of RDs remain unreported, undiagnosed, and untreated. To frame policies regarding RDs, it is crucial to understand the current level of RD awareness among healthcare professional and researchers, as they are key stakeholders in diagnosis, treatment, policy making, and drug development. We conducted an exploratory survey to understand the current level of RD awareness among healthcare professionals and researchers based on identification of an RD, time for diagnosis, treatment options, and relationship with family history and geographic location. We noted that our respondents have considerably low level of RD awareness. They correctly identified the importance of family history but failed to realize the association with geographic location. After presenting the survey findings, we have made recommendation to improve RD awareness in India. Our findings will be helpful to design awareness campaigns and frame relevant policies.

scientific communication and education↗