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Balkrishna, A.

Publications and source records attributed to Balkrishna, A..

7 recordsLinked to original sources

Assessment of Antibiotic Resistance Profiles in Cultivable Coliform Organisms Isolated from Ganga River Waters Across the Upper, Middle, and Lower Ganga Stretch

Antibiotic resistance in coliform organisms isolated from the Ganga River waters across its Upper, Middle, and Lower stretches was assessed. The study revealed varying levels of antibiotic resistance among the coliform isolates, with differences observed across the different stretches of the river. In the Upper stretch, sites like Devprayag and Gangotri exhibited high levels of E. coli contamination, with corresponding moderate antibiotic resistance (MAR) values. In the Middle stretch, sites like Farrukhabad and Bithoor showed lower E. coli contamination compared to the Upper stretch but had higher MAR values. The Lower stretch, particularly sites like Revelganj and Bahachoki in Bihar, displayed significantly elevated E. coli contamination levels and MAR values. These findings underscore the presence of antibiotic-resistant coliform organisms in different segments of the Ganga River, emphasizing the need for targeted interventions to mitigate antibiotic resistance spread in this vital water resource. Understanding the antibiotic resistance profiles at specific sites along the Ganga River can inform strategies to preserve water quality and protect public health in regions dependent on this iconic river.

microbiology↗

Evaluation Of Suitability Analysis of Gangetic Water from Upper, Middle, And Lower Ganga Rivers

This study assesses the suitability of water from distinct segments of the Ganga River in India for drinking, irrigation, and industrial purposes, utilizing various indices. Conducted in MAR-MAY 2023, water samples were collected from multiple sites along the river, and categorized into upper, middle, and lower stretches. The findings revealed differing levels of suitability for various purposes among the sampled sites within each stretch. Parameters for drinking water quality generally adhered to specified limits across all stretches, except for E. coli, emphasizing the importance of disinfection measures. Moreover, the study evaluated irrigation suitability, with most sites falling within the good to excellent range, albeit some exhibiting elevated sodium levels, particularly in the lower stretch. Analysis of major ion abundance revealed an alkaline nature dominated by sodium and potassium ions over calcium and magnesium ions across all stretches. Heavy metal concentrations were either within specified limits or absent, indicating minimal industrial pollution. However, caution is warranted regarding industrial applications due to water aggressiveness, as suggested by indices such as the Langelier saturation index and Ryznar stability index, which hint at potential corrosion issues. Water quality in the Ganga River in India exhibited variability across the upper, middle, and lower stretches, with each segment presenting unique characteristics and suitability levels. Continuous monitoring and control measures, especially concerning the most probable number (MPN) of contaminants, are imperative to ensure the sustainability of water quality and meet the diverse usage needs along the river. The evaluation of Gangetic water from the Upper, Middle, and Lower Ganga Rivers is crucial for understanding water quality in one of Indias vital river basins. This study assesses physical, chemical, and biological parameters to determine suitability for drinking, irrigation, and aquatic life. Factors like pH, dissolved oxygen, turbidity, nutrients, heavy metals, and microbial load are analyzed to reveal water quality and potential risks. Temporal and spatial variations, influenced by natural processes and human activities, are explored. The findings aid in sustainable water management and conservation in the Gangetic basin, addressing the urgent need to safeguard this critical resource.

microbiology↗

Unraveling the Interplay between Phytochemicals and Rhizosphere Bacteria in Ficus racemosa

Plant-microbe interactions in the rhizosphere play crucial roles in plant health and ecosystem functioning. Despite extensive research, gaps persist in understanding the relationship between phytochemicals and rhizosphere bacteria, particularly in species like Ficus racemosa. This study aims to investigate this interplay by characterizing the diversity and abundance of rhizosphere bacteria in Ficus racemosa and assessing the influence of phytochemical composition on microbial community structure and function. Through a multidisciplinary approach integrating microbiology and phytochemistry, our findings contribute to a holistic understanding of rhizosphere ecology and inform strategies for optimizing plant-microbe interactions in agricultural and environmental contexts.

microbiology↗

Anti-oxidant Response of Lipidom Modulates Lipid Metabolism in Caenorhabditis elegans and in OxLDL-Induced Human Macrophages by Tuning Inflammatory Mediators

Atherosclerosis is the main pathological process of most cardiovascular diseases. It can begin early in life and may remain latent and asymptomatic for an extended period before its clinical manifestation. Lipidom, an ayurvedic prescription medicine, contains five herbal constituents with reported anti-inflammatory, anti-oxidant and lipid lowering properties. The present study is aimed to characterize the pharmacological potentials of Lipidom. The phytochemical analysis of Lipidom was performed on high performance liquid chromatography (HPLC) platform. Lipidom was evaluated for cytosafety, NF-{kappa}B activity, IL-1{beta} and MCP-1 levels, modulation of NLRP3 pathway, ROS generation, lipid accumulation and gene expression in oxidized LDL stimulated THP1 macrophages. Furthermore, assessment of Lipidom was also done in the in-vivo Caenorhabditis elegans model. Analysis of brood size, % adult, lipid accumulation, triglyceride levels, MDA formation, SOD-3 levels and gene expression was performed in C. elegans. Lipidom treatment significantly reduced the inflammatory markers, lipid accumulation, oxidative stress and normalized genes involved in atherosclerosis development in THP1 macrophages. Lipidom treated C. elegans showed a significant decline in the lipid accumulation and oxidative stress. Lipidom showed a multifaceted approach in modulating the mediators responsible for development and progression of atherosclerosis.

pharmacology and toxicology↗

LC/MS-QToF Profiling, Anti-Diabetic and Anti-Adipogenic potential of Divya MadhuKalp: A Novel Herbo-mineral Formulation

The incidence rate of diabetes mellitus is increasing worldwide. Herbal formulations have recently gained importance as an alternative therapeutic option in controlling diabetes without causing any side effects. In the present study, we have demonstrated maintenance of glycemic homeostasis and anti-adipogenic potential of a herbo-mineral formulation Divya MadhuKalp (DMK). Initially, we evaluated the presence of bioactive compounds in DMK using LC/MS-QToF analysis. In-vitro analysis of DMK in L6 (skeletal muscle) cells showed a significant increase in cellular glucose uptake. Similarly, a human equivalent dose of DMK significantly reduced blood glucose level in normoglycemic and oral glucose tolerance rat model. DMK extract also inhibited formation of advanced glycation end product and showed anti--glucosidase activity. Further analysis of DMK in 3T3 L1 pre-adipocytes demonstrated anti-adipogenic activity through reduction in intracellular lipid accumulation and triglyceride contents along with downregulation of major adipogenic transcriptional factors (PPAR-{gamma} and C/EBP) and, adipocytes marker genes (LPL, AP2 and adiponectin). In conclusion, DMK exhibited anti-diabetic and anti-adipogenic activities by synergistic effect of its bioactive compounds and can be considered as a potent herbo-mineral formulation for treating metabolic diseases.

cell biology↗

Cellular internalization of Godanti Bhasma (anhydrous CaSO4) induces massive cytoplasmic reversible vacuolation and survival response of mammalian cells.

Bhasmas are Ayurvedic herbo-mineral formulations that have been used since ancient times for therapeutic benefits. Godanti Bhasma (GB) is an anhydrous calcium sulfate preparation processed by heating of gypsum powder with herbal extracts. Thermo-transformation of gypsum into the anhydrous GB was confirmed by Raman and FT-IR spectroscopy. GB particle showed size range of 0.5-5 {micro}m and neutral surface charge. Exposure to mammalian cells with GB particles showed massive vacuolation in their cytoplasm. Interestingly, no vacuolation was observed with parent gypsum particle. The result indicated that the cytoplasmic vacuolation by GB was due to its unique physicochemical property obtained during the thermo-transformation of gypsum. Using lysosomal inhibitors Bafilomycin A1 (BFA1) and Chloroquine (CQ), the process of vacuole formation was suppressed indicating GB induced vacuolation require acidic environment. The GB induced vacuolation was also found to follow dose and time dependent manner. Vacuolation often accompany with the sign of cell death whereas, in our study, massive vacuolation by GB did not induce any cell death. Moreover, GB treated cells survive with massive vacuolar process, which was reversed following post-treatment with vacuole inhibitors in GB treated cells, suggesting normal vacuolar function is essential for cell survival. Treatment of cells with GB was also found to induce translocation of LC3 protein from the nucleus to vacuolar membrane, indicating LC3 associated phagocytosis (LAP) is involved in the vacuolar process. Interestingly, the LAP function was found to be reversed in the cells treated with vacuole inhibitors. Our results provide a mechanistic correlation with GB induced vacuolation and associated LAP function, essential for cell survival. O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=164 SRC="FIGDIR/small/930594v3_ufig1.gif" ALT="Figure 1"> View larger version (27K): org.highwire.dtl.DTLVardef@169ed89org.highwire.dtl.DTLVardef@169c339org.highwire.dtl.DTLVardef@900004org.highwire.dtl.DTLVardef@1f13823_HPS_FORMAT_FIGEXP M_FIG C_FIG

cell biology↗

Formulation, Evaluation and Antibacterial Efficiency of water-based herbal Hand Sanitizer Gel

Hands are the primary mode of transmission of microbes and infections. Hand-washing is critical in food production, food service and also important in healthcare setting, homes and day care preparations. The present research was aimed to evaluate the antibacterial efficacy of various herbal oils such as Cinnamon oil, Eucalyptus oil, menthol oil and lavender oil and found that cinnamon oil showed better antibacterial activity. Also the research was carried out to formulate and evaluate the poly herbal Hand wash gel containing Azadirachta indica, Ocimum sanctum and Citrus limon extracts. The anti-microbial activity of the formulated herbal hand wash gel was tested against Escherichia coli, Staphylococcus aureus and Salmonella by spread plate techniques and the results obtained were compared with commercial antibacterial standards. Also the efficiency was checked by using the hand wash gel on volunteers. The results from the present work suggest and support the incorporation and utilization of herbs in the formulations to give better effect.

microbiology↗