Biology subjects

Bakhshi, B.

Publications and source records attributed to Bakhshi, B..

3 recordsLinked to original sources

bioRxiv2021

Identification of promising oilseed rape genotypes for the tropical regions of Iran using multivariate analysis

producing new adapted oilseed rape cultivars among the available resources of rapeseed would be a valuable method to increase the cultivar diversity in the tropical regions. Low adaptable and high yield cultivars resources of oilseed rapes are now available in the tropical regions of Iran. The current study aimed to identify new high yield and adaptable genotypes adaptable across various tropical regions. To this end, 20 new genotypes and a check variety (Dalgan) were cultivated in the tropical regions of Iran based on a randomized complete block design (RCBD) with three replications during the 2019 to 2020 cropping season. The experimental sites are composed of five locations, including Gorgan, Sari, Rasht, Borazjan and Zabol. During the growth season, several phenological and quantitative traits were recorded. Combined ANOVA revealed significant genotype by environment interaction for all studied quantitative traits. Days to start flowering and days to end flowering showed the highest heritability. Correlation analysis showed a significant positive relationship between yield and flowering period, the number of sub-branches and also the number of pods per plant, but a negative and significant correlation with the days to maturity. Path analysis showed that the days to maturity had the most negative direct effect on yield and the days to start flowering, while the number of sub-branches had the most positive direct effect on yield. Canonical correlation showed that yield is correlated positively with phenological traits. The principal component analysis showed that the two first components covered 68.07% of all data variations which 12 genotypes were correlated with these two components. Cluster analysis categorized evaluated genotypes into three main groups. Finally, eight genotypes (from class 2 of the cluster) were selected in the current study, which had high yield and adaptability in the tropical regions of Iran.

genetics↗

bioRxiv2019

Distribution and characterization of Aegilops cylindrica species from Iran

Jointed goatgrass (Aegilops cylindrica Host; 2n = 4x = 28, CcCcDcDc) is a tetraploid remote relative of bread wheat (Triticum aestivum L; 2n=6x=42, AABBDD) with 2 genomes and 28 chromosomes. The diversity center of this species is in the Fertile Crescent and in central Asia and could also be found in many places in Iran. In this experiment, 359 accessions provided by National Plant Gene Bank of Iran (NPGBI) were used. Based on the geographical distribution, the highest distribution of Ae. cylindrica are from North, West and North West regions of Iran. The distribution data of Ae. cylindrica showed that the distribution centers in Iran are more than those reported in previous studies in Iran. Chromosome counting showed that all Ae. cylindrica accessions are tetraploid (2n=4x=28). Results of factor analysis for 9 morphological chromosome traits showed that karyotypic variation within accessions are related to the length of chromosomes and there is difference between accessions for their total chromosome length, but the karyotype of different accessions were almost the same for the symmetry. Low coefficient of variation in morphological traits as well as symmetric karyotypes of Ae. cylindrica species observed in this study could lead us to more confidently say that Ae. cylindrica could be a recently evolved species among remote relatives of bread wheat.

plant biology↗

bioRxiv2019

Whole Aegilops tauschii transcriptome investigation revealed nine novel miRNAs involved in stress response

Aegilops tauschii is wild relative of bread wheat. This species has been reported as donor of bread wheat D genome. There are also many reports that mentioned Ae. tauschii importance in biotic and abiotic stress resistance. Thus, it is important to investigate regulatory mechanisms involved in Ae. tauschii stress tolerance. MiRNAs have been reported as essential regulatory elements in stress response. In this study, using ESTs/TSAs databases nine novel miRNA stem-loops have been identified including ata-miRNovel_1 to 9. MiR156, miR168, miR169, miR319, miR397 and miR530 are some miRNA families that their mature miRNAs could be matched with these novel stem-loop miRNAs. We have also observed wheat ESTs/TSAs as similar as Ae. tauschii ESTs/TSAs that could produce new miRNA stem-loops in wheat. These novel miRNA stem-loops could play important role in transcription regulation involved in stress response.

molecular biology↗