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Bakhshi, B.

Publications and source records attributed to Bakhshi, B..

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Distribution and characterization of Aegilops cylindrica species from Iran

Jointed goatgrass (Aegilops cylindrica Host; 2n = 4x = 28, CcCcDcDc) is a tetraploid remote relative of bread wheat (Triticum aestivum L; 2n=6x=42, AABBDD) with 2 genomes and 28 chromosomes. The diversity center of this species is in the Fertile Crescent and in central Asia and could also be found in many places in Iran. In this experiment, 359 accessions provided by National Plant Gene Bank of Iran (NPGBI) were used. Based on the geographical distribution, the highest distribution of Ae. cylindrica are from North, West and North West regions of Iran. The distribution data of Ae. cylindrica showed that the distribution centers in Iran are more than those reported in previous studies in Iran. Chromosome counting showed that all Ae. cylindrica accessions are tetraploid (2n=4x=28). Results of factor analysis for 9 morphological chromosome traits showed that karyotypic variation within accessions are related to the length of chromosomes and there is difference between accessions for their total chromosome length, but the karyotype of different accessions were almost the same for the symmetry. Low coefficient of variation in morphological traits as well as symmetric karyotypes of Ae. cylindrica species observed in this study could lead us to more confidently say that Ae. cylindrica could be a recently evolved species among remote relatives of bread wheat.

plant biology

Whole Aegilops tauschii transcriptome investigation revealed nine novel miRNAs involved in stress response

Aegilops tauschii is wild relative of bread wheat. This species has been reported as donor of bread wheat D genome. There are also many reports that mentioned Ae. tauschii importance in biotic and abiotic stress resistance. Thus, it is important to investigate regulatory mechanisms involved in Ae. tauschii stress tolerance. MiRNAs have been reported as essential regulatory elements in stress response. In this study, using ESTs/TSAs databases nine novel miRNA stem-loops have been identified including ata-miRNovel_1 to 9. MiR156, miR168, miR169, miR319, miR397 and miR530 are some miRNA families that their mature miRNAs could be matched with these novel stem-loop miRNAs. We have also observed wheat ESTs/TSAs as similar as Ae. tauschii ESTs/TSAs that could produce new miRNA stem-loops in wheat. These novel miRNA stem-loops could play important role in transcription regulation involved in stress response.

molecular biology