Ayaz, M. | bioRxiv Science

bioRxiv2026

Assessing the impact of gamma irradiation on key biological traits of peach fruit fly, Bactrocera zonata (Diptera: Tephritidae) under laboratory conditions

Bactrocera zonata (Saunders) (Diptera: Tephritidae) poses a significant threat to global fruit production due to its high reproductive capacity and broad host range. This study aimed to evaluate the effects of gamma irradiation on key biological parameters of B. zonata. Six-day-old pupae were exposed to irradiation doses of 0, 30, 40, 50, 60, and 70 Gy, and subsequent developmental and reproductive traits were assessed to determine the impact of irradiation. Post-irradiation results revealed a dose-dependent trend. Higher doses ([≥]50 Gy) significantly reduced adult emergence, increased the incidence of partially emerged or deformed adults, and shortened adult longevity. Reproductive potential was significantly impaired in males irradiated at 60 and 70 Gy when mated with un-irradiated females, resulting in a marked decline in both fecundity and egg hatchability. Females irradiated at doses [≥]50 Gy failed to produce eggs when paired with either irradiated or non-irradiated males, indicating a high level of radio-sensitivity in female flies. Additionally, several traits in the F1 generation such as pupal recovery, pupal size, and adult development exhibited significant abnormalities and suggesting that the effects of irradiation may be transmitted to the next generation. Sterility was highest in males irradiated at 60 and 70 Gy, while females exhibited complete sterility at doses exceeding 40 Gy. The findings indicate that a dose of 70 Gy may be optimal for effective sterility induction in B. zonata. However, further detailed studies are required to standardize this dose, incorporating rigorous quality control measures to optimize its application in sterile insect technique (SIT) programs.

zoology↗

bioRxiv2016

Mapping Autosomal Recessive Intellectual Disability: Combined Microarray and Exome Sequencing Identifies 26 Novel Candidate Genes in 192 Consanguineous Families

Approximately 1% of the global population is affected by intellectual disability (ID), and the majority receive no molecular diagnosis. Previous studies have indicated high levels of genetic heterogeneity, with estimates of more than 2500 autosomal ID genes, the majority of which are autosomal recessive (AR). Here, we combined microarray genotyping, homozygosity-by-descent (HBD) mapping, copy number variation (CNV) analysis, and whole exome sequencing (WES) to identify disease genes/mutations in 192 multiplex Pakistani and Iranian consanguineous families with non-syndromic ID. We identified definite or candidate mutations (or CNVs) in 51% of families in 72 different genes, including 26 not previously reported for ARID. The new ARID genes include nine with loss-of-function mutations (ABI2, MAPK8, MPDZ, PIDD1, SLAIN1, TBC1D23, TRAPPC6B, UBA7, and USP44), and missense mutations include the first reports of variants in BDNF or TET1 associated with ID. The genes identified also showed overlap with de novo gene sets for other neuropsychiatric disorders. Transcriptional studies showed prominent expression in the prenatal brain. The high yield of AR mutations for ID indicated that this approach has excellent clinical potential and should inform clinical diagnostics, including clinical whole exome and genome sequencing, for populations in which consanguinity is common. As with other AR disorders, the relevance will also apply to outbred populations.

genetics↗