Anupoju, G. R. | bioRxiv Science

bioRxiv2026

Unlocking the potential of Gordonia rubripertincta in syngas fermentation for carbon monoxide bioconversion into carotenoids

Fermentation of C1 gases is an emerging technology where waste gases are bio converted into value-added products. This study navigates the gas fermentation potential of Gordonia rubripertincta to produce carotenoids. The crucial carbon monoxide dehydrogenase (CODH) enzyme, necessary for gas uptake by the microbe, was found to be present in G. rubripertincta through blastp on NCBI website. The organism was then used for gas fermentation experiments in a continuous stirred tank reactor (CSTR) in different modes of reactor operation resulting in the production of about 500 mg pigment/g WCW (wet cell weight). Two important reactor parameters, molybdenum content and pH, were optimized for enhanced carotenoid production. Overall, G. rubripertincta was observed to be an efficient candidate organism for C1 gas fermentation. KEY HIGHLIGHTSO_LIGordonia rubripertincta synthesises aerobic carbon monoxide dehydrogenase enzyme. C_LIO_LIIt is a potential gas fermenting microbe that gives carotenoids as product. C_LIO_LIThe gas uptake efficiency of the microbe is more in fed-batch discontinued mode. C_LIO_LIIn FB-D, the resultant carotenoids are 500+9 mg/g wet cell weight (WCW). C_LIO_LIMo/pH of 20 mg/7.0 resulted in highest carotenoids, i.e., 134+41 mg/g WCW. C_LI GRAPHICAL ABSTRACT O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=87 SRC="FIGDIR/small/722808v1_ufig1.gif" ALT="Figure 1"> View larger version (28K): org.highwire.dtl.DTLVardef@8b1185org.highwire.dtl.DTLVardef@2b6f90org.highwire.dtl.DTLVardef@1a9697dorg.highwire.dtl.DTLVardef@14c9dc8_HPS_FORMAT_FIGEXP M_FIG C_FIG

bioengineering↗

bioRxiv2025

Caroten-omics of pigments synthesised by Gordonia rubropertincta through High Resolution Mass Spectrometry and in-silico programming approach

Carotenoids are tetraterpenoid pigments synthesised by microorganisms, plants and present in some animals. They are metabolites of importance in research and industry. The present study aimed to analyse the High Resolution Mass Spectrometry (HRMS) experimental data through R programming and identify all the carotenoids that Gordonia rubropertincta produces. The growth rate of the bacterium was 0.24 {+/-} 0.132 h-1, produced about 140 {+/-} 20 mg carotenoids/g wet cell weight (WCW) on extraction with methanol and the extract was subjected to HRMS. The number of carotenoids the bacterium synthesizes was identified to be 93 and the concentration of few carotenoids at particular time was pinpointed using R. The distinctive features of the bacterium like high number of carotenoids synthesis through various pathways, their fluctuation with respect to time, storage of carotenoids in cell cytoplasm, and its auto-fluorescent property were also discovered.

microbiology↗