bioRxiv Science⌕ Search

Biology subjects

Anandan, R.

Publications and source records attributed to Anandan, R..

3 recordsLinked to original sources

Microbial Production Of Ellagic Acid From Mango Pulp Processing Waste

Ellagic acid has gained momentum recently due to its various properties like anti-mutagenic, anti-carcinogenic, anti-oxidant, and anti-viral and many other benefits to human health. The present study focused on the microbial production of ellagic acid from mango pulp processing industrial waste an alternate method for conventional chemical extraction. Our experiments demonstrated that the 100 g/ml of ellagic acid was produced by Micrococcus luteus from 9% of mango pulp waste and the optimization of ellagic acid production with Pontecorvo medium supplemented with 5.0 g of ellagitannin has yielded 37.80 {+/-} 0.30 mg/g at pH 5.0, temperature 30 {degrees}C, ammonium nitrate (nitrogen source), glucose (carbon source), with 1.5% of inoculums after 24 h of incubation. Ellagic acid synthesized was further confirmed with the standard ellagic acid. Applications like drought resistant in plants, anti-microbial activity, anti-parasitic activity and anti-cancer activities have been proven. Ellagic acid exhibited potential applications and further research in product development is promising.

microbiology↗

Bioconversion of Mango Pulp Industrial Waste into Ellagic acid Using Aspergillus niger

Ellagic acid was considered as the potential bioactive compound with many therapeutical applications. Bioconversion of tannin present in the mango pulp processing waste in to ellagic acid using fungi would be better alternate than the chemical as well as extraction from plant sources. A total of three different fungi were isolated from the soil sample and it was confirmed as Aspergillus niger. Further, the isolated strains of A. niger were identified to produce ellagic acid from ellagitannin of mango waste. Quantification of the ellagic acid production was carried out by solid-state fermentation using 3% of mango waste as substrate. Ellagic acid enzyme activity was calculated and found to be 17.6 U ml-1 The ellagic acid production was optimized to fix the various factors, that is, pH and temperature, nitrogen and carbon source. The maximum production (200 g/g) of ellagic acid was achieved at pH 5.5, temperature 30 {degrees}C, Ammonium nitrate as nitrogen source, 0.2% of NaCl and carbon source (0.2% of sugar) with 3% of mango pulp waste. Ellagic acid produced was characterized by UV-vis spectrophotometer and by FT-IR analysis.

microbiology↗

Efficacy of Methylobacterium oryzae Supplemented Sargassum wightii Seaweed Liquid Fertilizer on Chilly and Tomato Plant Growth

Liquid seaweed fertilizer gaining interest due to their use in crop productivity. The present study was designed to evaluate the impact of Methylobacterium oryzae amended liquid seaweed fertilizer on domestic plants like chilly and tomato. The Sargassum wightii methanolic extract was maximum (137 mg/g) among other solvent tested. Extract showed 0.456mg/mL, 1.587 mg/mL, 0.78 mg/g, 5.27 mg/g, 0.63 mg/mL, 0.98 mg/mL, 0.285 g/mL and 0.546 g/mL of total phenolic, total flavonoids, total chlorophyll, total carotenoids, total protein, total carbohydrates, total lipids and total aminoacids respectively. The maximum survival of Methylobacterium oryzae was observed at 40 % of Sargassum wightii SLF extract with 3% Methylobacterium oryzae culture concentration and has viability of 750 x 106 CFU/l after 6 months. Impact of foliar sprayed liquid fertilizer on the chlorophyll, internode and shoot length had been promising over seed soaked.

plant biology↗