Almato-Bellavista, M. | bioRxiv Science

bioRxiv2024

Emo-FilM: A multimodal dataset for affective neuroscience using naturalistic stimuli

The extensive Emo-FilM dataset stands for Emotion research using Films and fMRI in healthy participants. This dataset includes detailed emotion annotations by 44 raters for 14 short films with a combined duration of over 2[1/2] hours, as well as recordings of respiration, heart rate, and functional magnetic resonance imaging (fMRI) from a different sample of 30 individuals watching the same films. The detailed annotations of experienced emotion evaluated 50 items including ratings of discrete emotions and emotion components from the domains of appraisal, motivation, motor expression, physiological response, and feeling. Quality assessment for the behavioural data shows a mean inter-rater agreement of 0.38. The parallel fMRI data was acquired at 3 Tesla in four sessions, accompanied with a high-resolution structural (T1) and resting state fMRI scans for each participant. Physiological recordings during fMRI included heart rate, respiration, and electrodermal activity (EDA). Quality assessment indicators confirm acceptable quality of the MRI data. This dataset is designed, but not limited, to studying the dynamic neural processes involved in emotion experience. A particular strength of this data is the high temporal resolution of behavioural annotations, as well as the inclusion of a validation study in the fMRI sample. This high-quality behavioural data in combination with continuous physiological and MRI measurements makes this dataset a treasure trove for researching human emotion in response to naturalistic stimulation in a multimodal framework.

neuroscience↗

bioRxiv2023

Human intestinal organoids with an autologoustissue-resident immune compartment

The intimate relationship between the epithelium and the immune system is crucial for maintaining tissue homeostasis, with perturbations in epithelial-immune interactions linked to autoimmune disease and cancer. Whereas stem cell-derived organoids are powerful models of tissue-specific epithelial function, these structures lack tissue-resident immune cells that are essential for capturing organ-level processes. We describe human intestinal immuno-organoids (IIOs), formed through self-organization of epithelial organoids and autologous tissue-resident lymphocytes (TRMs), a portion of which integrate within the IIO epithelium and survey the barrier. IIO formation was driven by TRM migration and interaction with epithelial cells, as orchestrated by TRM-enriched transcriptomic programs governing cell motility and epithelial inspection. We combined IIOs and single-cell transcriptomics to investigate intestinal inflammation triggered by cancer-targeting biologics in patients, and found that the system recapitulates clinical outcomes and the underlying cellular mechanisms. Inflammation was associated with the emergence of an activated population of CD8+ T cells, which progressively acquired intraepithelial and cytotoxic features. The appearance of this effector population was preceded and likely mediated by a Th1-like CD4+ population, which initially displayed a cytokine-producing character and subsequently became cytotoxic itself. A system amenable to direct perturbation and interrogation, IIOs allowed us to identify the Rho pathway as a novel target for mitigating immunotherapy-associated intestinal inflammation. Given that they recapitulate both the phenotypic outcomes and the underlying inter-lineage immune interactions, IIOs can be used to broadly study tissue-resident immune responses in the context of tumorigenesis, infectious and autoimmune diseases.

immunology↗