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Allen, B. J.

Publications and source records attributed to Allen, B. J..

2 recordsLinked to original sources

DeepDiveR - A software for deep learning estimation of palaeodiversity from fossil occurrences

O_LIThe incompleteness of the fossil record, in particular variation in preservation and sampling through space and time, presents a barrier to estimating changes in biodiversity which standard statistical methods struggle to account for. C_LIO_LIHere we present DeepDiveR, an R package for the DeepDive program enabling estimation of biodiversity from fossil occurrence data. The method uses a simulation-trained deep neural network to generate predictions of biodiversity change through time, while accounting for temporal, spatial and taxonomic heterogeneities in preservation. C_LIO_LIDeepDiveR can be readily used to explore the extinct biodiversity of different clades. We demonstrate the pipeline to build and customise analyses, including consideration of changes in biogeography. We also further develop the model to integrate information about modern diversity in the case of extant clades and introduce a function that automatically adjusts the parameterization of the simulations to generate training data that reflect the distribution of empirical datasets. C_LIO_LITo demonstrate the software, we analyse the fossil record of the order Carnivora through the Cenozoic, finding a peak in diversity in the Late Miocene and a 37% species loss since the Pleistocene. Our implementation includes the generation summary statistics and plots that allow for an evaluation of the model performance and diversity estimations and a configuration file that captures all parameters required to guarantee the full reproducibility of the results. C_LI

paleontology↗

A genome sequence for the threatened whitebark pine

Whitebark pine (WBP, Pinus albicaulis) is a white pine of subalpine regions in western contiguous US and Canada. WBP has become critically threatened throughout a significant part of its natural range due to mortality from the introduced fungal pathogen white pine blister rust (WPBR, Cronartium ribicola) and additional threats from mountain pine beetle (Dendroctonus ponderosae), wildfire, and maladaptation due to changing climate. Vast acreages of WBP have suffered nearly complete mortality. Genomic technologies can contribute to a faster, more cost-effective approach to the traditional practices of identifying disease-resistant, climate-adapted seed sources for restoration. With deep-coverage Illumina short-reads of haploid megametophyte tissue and Oxford Nanopore long-reads of diploid needle tissue, followed by a hybrid, multistep assembly approach, we produced a final assembly containing 27.6 Gbp of sequence in 92,740 contigs (N50 537,007 bp) and 34,716 scaffolds (N50 2.0 Gbp). Approximately 87.2% (24.0 Gbp) of total sequence was placed on the twelve WBP chromosomes. Annotation yielded 25,362 protein-coding genes, and over 77% of the genome was characterized as repeats. WBP has demonstrated the greatest variation in resistance to WPBR among the North American white pines. Candidate genes for quantitative resistance include disease resistance genes known as nucleotide-binding leucine-rich-repeat receptors (NLRs). A combination of protein domain alignments and direct genome scanning was employed to fully describe the three subclasses of NLRs (TNL, CNL, RNL). Our high-quality reference sequence and annotation provide a marked improvement in NLR identification compared to previous assessments that leveraged de novo assembled transcriptomes.

genomics↗