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Akyol, S.

Publications and source records attributed to Akyol, S..

2 recordsLinked to original sources
bioRxiv2025

MODERATE 3D ORBITAL SHAKING MITIGATES MATERNAL SEPARATION-INDUCED NEURODEVELOPMENTAL IMPAIRMENTS IN RATS

Maternal separation during early life is a well-established model for inducing neurodevelopmental impairments. Mechanical stimulation by passive movement, such as three-dimensional orbital shaking (3D-OS), has been suggested as a neuroprotective intervention in cell and tissue models, but its effects in neonatal animals remain unknown. We investigated whether 3D-OS applied during the postnatal period could attenuate neurogenesis impairments and cognitive deficits induced by maternal separation in rats. Eighteen newborn Sprague Dawley rats were assigned to control (C), maternal separation (MS), or maternal separation plus orbital shaking (MSOS) groups. Maternal separation was performed 3 h/day between postnatal days 2-21; MSOS additionally received 3D-OS stimulation (25 rpm, 3 h/day). Behavioural tests (Open Field, Novel Object Recognition, Morris Water Maze, Passive Avoidance), combined with RT-qPCR, Western blot, histology, and immunohistochemistry were conducted. MSOS rats displayed improved spatial learning in the early phases of the Morris Water Maze and recovered retention memory in Passive Avoidance compared to MS. In contrast, Open Field and Novel Object Recognition tests revealed no differences, and Western blot did not detect significant protein changes. Histological analysis showed reduced neuronal loss and vacuolisation in hippocampal and cortical regions of MSOS rats. RT-qPCR demonstrated reduced hippocampal FGF2 and FGFR1 expression in MS, partially restored by 3D-OS, while immunohistochemistry indicated increased FGFR2 and decreased FGFR1 in MSOS. Early-life 3D-OS administration alleviated maternal separation-induced cognitive decline and structural impairments, and enhanced hippocampal plasticity markers, providing preclinical evidence that moderate mechanical stimulation may support brain development and exert neuroprotective effects against early-life stress. HighlightsO_LIEarly-life 3D-OS mitigated maternal separation-induced deficits. C_LIO_LIImproved performance in Morris Water Maze and Passive Avoidance tasks. C_LIO_LINo significant effects observed in OFT, NOR, or Western blot. C_LIO_LI3D-OS restored hippocampal FGF-2 and FGFR1 mRNA levels in stressed rats. C_LIO_LIGentle mechanical stimulation may support early brain development. C_LI

animal behavior and cognition↗
bioRxiv2024

Interlaboratory comparison of standardised metabolomics and lipidomics analyses in human and rodent blood using the MxP(R) Quant 500 kit

Metabolomics and lipidomics are pivotal in understanding phenotypic variations beyond genomics. However, quantification and comparability of mass spectrometry (MS)-derived data are challenging. Standardised assays can enhance data comparability, enabling applications in multi-center epidemiological and clinical studies. Here we evaluated the performance and reproducibility of the MxP(R) Quant 500 kit across 14 laboratories. The kit allows quantification of 634 different metabolites from 26 compound classes using triple quadrupole MS. Each laboratory analysed twelve samples, including human plasma and serum, lipaemic plasma, NIST SRM 1950, and mouse and rat plasma, in triplicates. 505 out of the 634 metabolites were measurable above the limit of detection in all laboratories, while eight metabolites were undetectable in our study. Out of the 505 metabolites, 412 were observed in both human and rodent samples. Overall, the kit exhibited high reproducibility with a median coefficient of variation (CV) of 14.3 %. CVs in NIST SRM 1950 reference plasma were below 25 % and 10 % for 494 and 138 metabolites, respectively. To facilitate further inspection of reproducibility for any compound, we provide detailed results from the in-depth evaluation of reproducibility across concentration ranges using Deming regression. Interlaboratory reproducibility was similar across sample types, with some species-, matrix-, and phenotype-specific differences due to variations in concentration ranges. Comparisons with previous studies on the performance of MS-based kits (including the AbsoluteIDQ p180 and the Lipidyzer) revealed good concordance of reproducibility results and measured absolute concentrations in NIST SRM 1950 for most metabolites, making the MxP(R) Quant 500 kit a relevant tool to apply metabolomics and lipidomics in multi-center studies.

biochemistry↗