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Akbarzadeh, A.

Publications and source records attributed to Akbarzadeh, A..

3 recordsLinked to original sources

Empowering human-like walking with a bio-inspired gait controller for an under-actuated torque- driven human model

Human gait simulation plays a crucial role in providing insights into various aspects of locomotion, such as diagnosing injuries and impairments, assessing abnormal gait patterns, and developing assistive and rehabilitation technologies. To achieve more realistic results in gait simulation, it is necessary to utilize a comprehensive model that closely replicates the kinematics and kinetics of the human gait pattern. OpenSim software provides anthropomorphic and anatomically accurate human skeletal structures that enable users to create personalized models for individuals to accurately replicate real human behavior. However, torque-driven models face challenges in balancing unactuated degrees of freedom during forward dynamic simulations. Adopting a bio-inspired strategy that ensures an individuals balance with a minimized energy expenditure, this paper proposes a gait controller for a torque-deriven OpenSim model to achieve a stable walking. The proposed controller takes a model-based approach to calculate a "Balance Equivalent Control Torque" and uses the concept of the hip-ankle strategy to distribute this balance torque to the lower-limb joints. To optimize the controller gains and the "Balance Distribution Coefficients", an interface is stablished between MATLAB and OpenSim that is capable of conducting controllable forward dynamic simulations. The simulation results demonstrate that the torque-driven model can walk naturally with joint torques suitably matching experimental data. The robustness of the bio-inspired gait controller is also assessed by applying a range of external forces on the upper body to disturb the model. The robustness analysis demonstrates the quick and effective balance recovery mechanism of the proposed bio-inspired controller.

bioengineering↗

Identification of hypoxia-specific biomarkers in salmonids using RNA-sequencing and validation using high-throughput qPCR

Identifying early gene expression responses to hypoxia (i.e., low dissolved oxygen) as a tool to assess the degree of exposure to this stressor is crucial for salmonids, because they are increasingly exposed to hypoxic stress due to anthropogenic habitat change, e.g., global warming, excessive nutrient loading, and persistent algal blooms. Our goal was to discover and validate gill gene expression biomarkers specific to the hypoxia response in salmonids across multi-stressor conditions. Gill tissue was collected from 24 freshwater juvenile Chinook salmon (Oncorhynchus tshawytscha), held in normoxia [dissolved oxygen (DO) > 8 mg L-1] and hypoxia (DO = 4{square}5 mg L-1) in 10 and 18{degrees}C temperatures for up to six days. RNA-sequencing (RNA-seq) was then used to discover 240 differentially expressed genes between hypoxic and normoxic conditions, but not affected by temperature. The most significantly differentially expressed genes had functional roles in the cell cycle and suppression of cell proliferation associated with hypoxic conditions. The most significant genes (n = 30) were selected for real-time qPCR assay development. These assays demonstrated a strong correlation (r = 0.88; p < 0.001) between the expression values from RNA-seq and the fold changes from qPCR. Further, qPCR of the 30 candidate hypoxia biomarkers was applied to an additional 322 Chinook salmon exposed to hypoxic and normoxic conditions to reveal the top biomarkers to define hypoxic stress. Multivariate analyses revealed that smolt stage, water salinity, and morbidity status were relevant factors to consider with the expression of these genes in relation to hypoxic stress. These hypoxia candidate genes will be put into application screening Chinook salmon to determine the identity of stressors impacting the fish.

genomics↗

Salmonid gene expression biomarkers indicative of physiological responses to changes in salinity, temperature, but not dissolved oxygen

An organisms ability to respond effectively to environmental change is critical to their survival. Yet, life stage and overall condition can dictate tolerance thresholds to heightened environmental stressors, such that stress may not be equally felt across individuals within a species. Environmental changes can induce transcriptional responses in an organism, some of which reflect generalized responses, and others are highly specific to the type of change being experienced. Thus, if transcriptional biomarkers specific to a heightened environmental stressor, even under multi-stressor impacts, can be identified, the biomarkers could be then applied in natural environments to determine when and where individuals are experiencing such stressors. Here, we validate candidate gill gene expression biomarkers by experimentally challenging juvenile Chinook salmon (Oncorhynchus tshawytscha). A sophisticated experimental set-up (four trials) manipulated salinity (freshwater, brackish water, and seawater), temperature (10, 14, and 18{degrees}C), and dissolved oxygen (normoxia and hypoxia), in all 18 possible combinations, for up to six days during the pre-smolt, smolt, and de-smolt life stages. In addition, we also describe the changes in juvenile behaviour, plasma variables, gill Na+/K+- ATPase (NKA) activity, body size, body morphology, and skin pigmentation associated with salinity, temperature, dissolved oxygen, mortality, and smolt status. We statistically identified biomarkers specific to salinity and temperature treatments, as well as mortality across multiple stressors and life stages. Similar biomarkers for the dissolved oxygen treatment could not be identified in the data and we discuss our next steps using an RNA-seq study. This work demonstrates the unique power of gene expression biomarkers to identify a specific stressor even under multi-stressor conditions.

genomics↗