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Ahmed, A. T.

Publications and source records attributed to Ahmed, A. T..

3 recordsLinked to original sources

Exploring the Effects of Glutathione Supplementation on Liver Enzymes and Serum Electrolyte Levels in Mice Exposed to 850-1900 MHz Mobile Phone Radiation: An Investigation into Radiative Stress and Potential Protective Measures

In an era where electronic advancements surge forward at an unprecedented pace, public apprehension surrounding the health ramifications of radio-frequency (RF) radiation intensifies. This study looked into the impact of chronic exposure to mobile phone radiation, coupled with glutathione supplementation, on serum lipid profiles and body weight in mice--a subject vital for understanding potential health risks in humans and animals respectively. Thirty-five male mice were divided into seven groups, each subjected to various mobile phone modes and glutathione treatments. Over five weeks, these mice were exposed to 300 missed calls daily, simulating real-world exposure scenarios. Different serum lipid parameters--triglycerides, cholesterol, high-density lipoprotein (HDL), and low-density lipoprotein (LDL)--were monitored and observed throughout the study period. Remarkably, triglycerides, cholesterol, and LDL levels exhibited no noteworthy statistical variances compared to the control group or among experimental cohorts exposed to diverse range of mobile phone radiation modes or glutathione supplements. However, we noted some little deviations in HDL levels, particularly in the silent and silent+glutathione groups. Moreover, we also noted an unexpected result in weight loss across all groups by week three, which was pronounced by week five, and notably pronounced in the glutathione-administered cohorts. This outcome hints at glutathiones complex role in mitigating some complications of mobile phone radiation exposure. In conclusion, the study suggests a potential interplay between glutathione supplementation, mobile phone radiation exposure, and HDL levels in mice--an avenue ripe for further exploration into the understanding of cellular response to modern technological exposures.

systems biology↗

Pilot Study of Metabolomic Clusters as State Markers of Major Depression and Outcomes to CBT Treatment

Major depressive disorder (MDD) is a common and disabling syndrome with multiple etiologies that is defined by clinically elicited signs and symptoms. In hopes of developing a list of candidate biological measures that reflect and relate closely to the severity of depressive symptoms, so-called \"state-dependent\" biomarkers of depression, this pilot study explored the biochemical underpinnings of treatment response to cognitive behavior therapy (CBT) in medication-freeMDD outpatients. Plasma samples were collected at baseline and week 12 from a subset of MDD patients (N=26) who completed a course of CBT treatment as part of the Predictors of Remission in Depression to Individual and Combined Treatments (PReDICT) study. Targeted metabolomic profiling using the the AbsoluteIDQ(R) p180 Kit and LC-MS identified eight \"co-expressed\" metabolomic modules. Of these eight, three were significantly associated with change in depressive symptoms over the course of the 12-weeks. Metabolites found to be most strongly correlated with change in depressive symptoms were branched chain amino acids, acylcarnitines, methionine sulfoxide, and -aminoadipic acid (negative correlations with symptom change) as well as several lipids, particularly the phosphatidlylcholines (positive correlation). These results implicate disturbed bioenergetics as an important state marker in the pathobiology of MDD. Exploratory analyses contrasting remitters to CBT versus those who failed the treatment further suggest these metabolites may serve as mediators of recovery during CBT treatment. Larger studies examining metabolomic change patterns in patients treated with pharmacotherapy or psychotherapy will be necessary to elucidate the biological underpinnings of MDD and the -specific biologies of treatment response.

neuroscience↗

Acylcarnitine Metabolomic Profiles Inform Clinically-Defined Major Depressive Phenotypes

BackgroundAcylcarnitines have important functions in mitochondrial energetics and {beta}-oxidation, and have been implicated to play a significant role in metabolic functions of the brain. This retrospective study examined whether plasma acylcarnitine profiles can help biochemically distinguish the three phenotypic subtypes of major depressive disorder (MDD)--(core depression (CD+), anxious depression (ANX+), and neurovegetative symptoms of melancholia (NVSM+))--following treatment with a selective serotonin reuptake inhibitor (SSRI).\n\nMethodsDepressed outpatients (n=240) from the Mayo Clinic Pharmacogenomics Research Network were treated with citalopram or escitalopram for eight weeks. Plasma samples collected at baseline and eight weeks post-treatment were profiled for multiple-, short-, medium- and long-chain acylcarnitine levels using AbsoluteIDQ(R)p180-Kit and LC-MS. Linear mixed effects models were used to examine whether acylcarnitine levels discriminate the clinical phenotypes at baseline or eight weeks post-treatment, and whether temporal changes in acylcarnitine profiles differ between groups.\n\nResultsAt baseline, significantly lower concentrations of short- and long-chain acylcarnitines were found in CD+ and NVSM+ compared to ANX+, and the short-chain acylcarnitines remained lower after eight weeks. At eight weeks, the medium- and long-chain acylcarnitines were significantly lower in NVSM+ compared to ANX+. Regarding changes baseline to week eight, short-chain acylcarnitine levels significantly increased in CD+ and ANX+, and medium- and long-chain acylcarnitines significantly decreased in NVSM+ and CD+.\n\nConclusionsIn depressed patients treated with SSRIs, {beta}-oxidation and mitochondrial energetics as evaluated by levels and changes in acylcarnitines may provide the biochemical basis of the clinical heterogeneity of MDD, especially when combined with clinical characteristics.

biochemistry↗