Reconstructing B cell receptor sequences from short-read single cell RNA-sequencing with BRAPeS
RNA-sequencing of single B cells provides simultaneous measurements of the cell state and its binding specificity. However, in order to uncover the latter further reconstruction of the B cell receptor (BCR) sequence is needed. We present BRAPeS, an algorithm for reconstructing BCRs from short-read paired-end single cell RNA-sequencing. BRAPeS is accurate and achieves a high success rate even at very short (25bp) read length, which can decrease the cost and increase the number of cells that can be analyzed compared to long reads. BRAPeS is publicly available in the following link: https://github.com/YosefLab/BRAPeS.
bioinformatics↗