Biology subjects

ALMERAS, L.

Publications and source records attributed to ALMERAS, L..

3 recordsLinked to original sources

bioRxiv2024

MALDI-TOF MS profiling and its contribution to mosquito-borne diseases: a systematic review

Mosquito-borne diseases are responsible for hundreds of thousands of deaths per year. The identification and control of the vectors that transmit pathogens to humans are crucial for disease prevention and management. Currently, morphological and molecular approaches are the standard methods used for vector identification, however, they present several limitations. In the last decade, matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) profiling emerged as an innovative technology in Biological Sciences and is now considered as a relevant tool for the identification of pathogens and arthropods. Beyond species identification, this tool can be relevant for the determination of different life traits of arthropod vectors. The purpose of the present systematic review was to highlight the contribution of MALDI-TOF MS to the surveillance and control of mosquito-borne diseases. Published articles from January 2003 to September 2023 were retrieved, considering different aspects of mosquito life traits which could be determinant in the transmission of diseases and vector management. The screening of scientific literature resulted in the selection of 54 published articles that assessed MALDI-TOF MS profiling to study various biological factors such species identification, life expectancy, gender, trophic preferences, microbiota and insecticide resistance. Although a large majority of the selected articles focused on species identification, the present review shows that MALDI-TOF MS profiling is promising for identifying various mosquito life traits with rapidity, high throughput capacity, reliability and low cost. The strength and weakness of this proteomic tool for vector control and surveillance are discussed.

ecology↗

bioRxiv2023

Potential of MALDI-TOF MS biotyping to detect deltamethrin resistance in the dengue vector Aedes aegypti.

Insecticide resistance in mosquitoes is spreading worldwide and represents a growing threat to vector control. Insecticide resistance is caused by different mechanisms including higher metabolic detoxication, target-site modification, reduced penetration and behavioral changes that are not easily detectable with simple diagnostic methods. Indeed, most molecular resistance diagnostic tools are costly and labor intensive and then difficult to use for routine monitoring of insecticide resistance. The present study aims to determine whether mosquito susceptibility status against the pyrethroid insecticides (mostly used for mosquito control) could be established by the protein signatures of legs and/or thoraxes submitted to MALDI-TOF Mass Spectrometry (MS). The quality of MS spectra for both body parts was controlled to avoid any bias due to unconformity protein profiling. The comparison of MS profiles from three inbreeds Ae. aegypti lines from French Guiana (IRF, IR03, IR13), with distinct deltamethrin resistance genotype / phenotype and the susceptible reference laboratory line BORA (French Polynesia), showed different protein signatures. On both body parts, the analysis of whole protein profiles revealed a singularity of BORA line compared to the three inbreeding lines from French Guiana origin, suggesting that the first criteria of differentiation is the geographical origin and/or the breeding history rather than the insecticide susceptibility profile. However, a deeper analysis of the protein profiles allowed to identify 10 and 11 discriminating peaks from leg and thorax spectra, respectively. Among them, a specific peak around 4870 Da was detected in legs and thoraxes of pyrethroid resistant lines compared to the susceptible counterparts hence suggesting that MS profiling may be promising to rapidly distinguish resistant and susceptible phenotypes. Further work is needed to confirm the nature of this peak as a deltamethrin resistant marker and to validate the routine use of MS profiling to track insecticide resistance in Ae. aegypti field populations. Author SummaryThe monitoring of mosquito insecticide resistance in local populations is essential to guide the choice of the vector control strategy. Current methods for resistance monitoring rely on biological, biochemical and molecular assays that all have their weakness. To circumvent these limitations, alternative methods have to be explored. In previous studies, MALDI-TOF MS profiling have proved it performance to classify mosquitoes at the species and sub-species levels. The present work aim was to assess whether MALDI-TOF MS profiling strategy could be useful for determination of mosquito susceptibility to the most used pyrethroid insecticide. In this way, four mosquito lines with distinct deltamethrin resistance genotype / phenotype were submitted to MS analysis. The accurate comparison of MS spectra showed different peak intensities between mosquitoes exhibiting different insecticide resistance profiles. Among discriminant peaks, one may be promising to detect insecticide-resistance mechanisms in public health mosquitoes. A better characterization of mosquito life traits will help countries to implement timely and locally adapted vector control interventions.

bioengineering↗

bioRxiv2020

Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts.

In French Guiana, the malaria, a parasitic infection transmitted by Anopheline mosquitoes, remains a disease of public health importance. To prevent malaria transmission, the main effective way remains Anopheles control. For an effective control, accurate Anopheles species identification is indispensable to distinguish malaria vectors from non-vectors. Although, morphological and molecular methods are largely used, an innovative tool, based on protein pattern comparisons, the Matrix Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) profiling, emerged this last decade for arthropod identification. However, the limited mosquito fauna diversity of reference MS spectra remains one of the main drawback for it large usage. The aim of the present study was then to create and to share reference MS spectra for the identification of French Guiana Anopheline species. A total of eight distinct Anopheles species, among which four are malaria vectors, were collected in 6 areas. To improve Anopheles identification, two body parts, legs and thoraxes, were independently submitted to MS for the creation of respective reference MS spectra database (DB). This study underlined that double checking by MS enhanced the Anopheles identification confidence and rate of reliable classification. The sharing of this reference MS spectra DB should made easier Anopheles species monitoring in endemic malaria area to help malaria vector control or elimination programs.

microbiology↗